cm. Subculture before cultures become 70 to 80% confluent or when cells reach 5 to 6 X10(4) viable cells/sq. cm. Incubate cultures at 37C. Interval: Every three days Medium Renewal: 2 to 3 times per week Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v)...
cm. Subculture before cultures become 70 to 80% confluent or when cells reach 5 to 6 X10(4) viable cells/sq. cm. Incubate cultures at 37C. Interval: Every three days Medium Renewal: 2 to 3 times per week Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v)...
Figure 1(b) Open in figure viewerPowerPoint Chemical structure of dammarane-type ginsenoside triterpenoids detected in the GE (P. quinquefolius). Regions R1–R3 consist of different attachments of sugar moieties and refer to the following abbreviations: Af: arabinofuranose, Ap: arabinopyranose, G:...
The media was chan- ged after every two days and the cells were subcultured upon 80% confluence. Cell viability assay The viability assay was carried out to determine any pos- sible adverse effects of extract, fractions and isolated com- pounds on the 3T3-L1 mouse pre-adipocyte cells. Cell...