Cell culture tested DMSO:ATCC 4-X Recommended serum: ATCC 30-2030 References: 886: Green H, Meuth M. An established pre-adipose cell line and its differentiation in culture. Cell 3: 127-133, 1974. PubMed: 442609
Mouse embryo 3T3-L1 cells were purchased from ATCC (Maryland, USA) and were cultured in DMEM medium (Thermo Fisher Scientific, USA) containing 10% fetal bovine serum (FBS) (Gibco, USA) at 37° C and 5% CO2. For adipogenesis, 3T3-L1 cells were incubated with a differentiation cocktail (D...
cunninghamii stem bark resin appreciably altered differentiation or adipogenesis in 3T3-L1 adipocytes. Table 2. Percentage glucose utilization by 3 T3-L1 adipocytes and Chang liver cells of different solvent extracts. Extract or treatmentGlucose utilization (%)# 3 T3-L1 adipocytesChang liver cells...
2.1. Cell culture and differentiation assays Mouse 3T3-L1 preadipocytes (ATCC, USA) were cultured in DMEM supplemented with 10% calf serum (CS) and incubated in a humidified atmosphere of 5% CO2 at 37 °C. For spontaneous differentiation, 3T3-L1 cells were cultured in DMEM with 10% FBS fo...
number may lead to an overall reduction in adipocyte tissue mass through adipocyte hyperplasia [23]. Preadipocytes are fibroblasts that are undifferentiated adipocytes and by reducing preadipocyte cell number or preventing differentiation may affect overall lipid acquisition [23] and potential disease risk...
There were proofs that elevated intracellular calcium played a vital role in suppressing adipocyte differentiation. The current findings demonstrated that CAG was a potential therapeutic candidate for alleviating obesity and hyperlipidemia. Introduction Adipose tissue is a vital organelle for energy storage. ...
We previously showed that expression levels of PKCδ splice variants PKCδI and PKCδII switched by day 4 of differentiation (2). Here we sought to evaluate the role of PKCδI during adipogenesis. Using primers specific to PKCδI, our real time qPCR results (Fig. 1) indicate that PKCδ...
Fig. 1.CQR-300 inhibits adipogeneic differentiation in 3T3-L1 cells. 3T3-L1 preadipocytes were induced to differentiate into adipocytes by indicated MDI solution in the presence orabsenceof CQR-300. (A) Cell viability after treatment with CQR-300 was determined byMTT assay. (B) Lipid droplets...
ZCE and ZCW significantly suppressed the expression of the adipocyte differentiation genes:C/EBPα,PPARγandADD-1(Fig. 3A). We also detected the expression ofPref-1gene and found that SA/nt cells have higher levels of gene expression than MA/nt cells. However,Pref-1expression was drastically...
2.1. Cell Culture and Induction of Preadipocyte Differentiation Mouse 3T3-L1 pre-adipocytes were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA) and were cultured in Dulbecco modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) at 37 °C ...