The natural plant polyphenol compound curcumin possesses the body weight lowering effect. We observed that curcumin attenuated miR-17-5p expression and stimulated Tcf7l2 expression in 3T3-L1 cells. These, along with the elevation of miR-17-5p expression in mouse epididymal fat tissue in response ...
鼠尾草酸(标准品)Carnosic acid质量规格:HPLC≥98%,标准品 大鼠髓样细胞触发性受体2(EM2)试剂盒Rat iggering receptor expressed on myeloid cells 2,EM2 ELISA kit 双去氧基姜黄素(标准品) Bisdemethoxycurcumin质量规格:HPLC≥98%,标准品 英文名称RatGhrelinELISAKit大鼠生长释放肽(Ghrelin)规格:96T/48T ...
Results The basal glucose uptake was not altered, and curcumin increased the insulin-stimulated glucose uptake in 3T3-L1 cells. Curcumin suppressed the transcription and secretion of TNF-α and IL-6 induced by palmitate in a concentration-dependent manner. Palmitate induced nuclear translocation of ...
为探讨柽柳黄素对3T3-L1脂肪细胞胰岛素抵抗的影响及AMPK信号通路的作用机制,本研究利用地塞米松诱导3T3-L1脂肪细胞,建立胰岛素抵抗模型,通过给药后检测细胞对Glu的摄取量和细胞内TG的含量,并采用qRT-PCR对AMPK信号通路中相关基因进行检测,利用分子对接软件对AMPK信号通路中相关蛋白进行分子对接,进一步采用Western...
Curcumin proved to be a strong candidate in the control of diabetes, since it was able to inhibit DPP-IV in Caco-2 cells (10 µmol for 24 h) and the phosphorylation of kinases regulated by extracellular signal (ERK) in C2C12 cells (5–45 µmol for 12 h), in addition to reducing...
Nickel oxide nanoparticles induce cytotoxicity, oxidative stress and apoptosis in cultured human cells that is abrogated by the dietary antioxidant curcumin Nickel oxide nanoparticles (NiO NPs) are increasingly utilized in a number of applications. However, little is known about the toxicity of NiO NPs...
Oil red O staining material (OROSM) was used as a marker of adipogenesis where the higher the OROSM value, the higher the lipid accumulation inside the cells. Basically, most of the GBR extracts significantly reduce (p < 0.05) lipid accumulation in 3T3-L1 adipocytes compared to control...
Curcumin inhibited 3T3-L1 adipocyte differentiation in dose-dependent manner by downregulating PPAR-γ expression. Curcumin was toxic to cells at a concentration of 30 μM. Curcumin also downregulated PPAR-α gene expression while not affecting Fabp4 gene expression indicating that fatty acid oxidation...
3T3-L1 preadipocytes (6 × 105 cells/well) were cultured in 6 cm cell dish plates for 24 h and then pretreated with marmin (50, 100, and 125 μg/mL) for 24 h. Cell treatment and sample action concentration were as the same as method 2.6. After incubation, the cell supernatants ...
3T3-L1 Pre-adipocytes as well as C57BL/6J mice on a high-fat diet (HFD) were used to test the anti-obesity effects of onion peel extract (OPE) in vitro and in vivo. OPE significantly decreased the lipids of 3T3-L1 cells and inhibited lipid accumulation by reducing the expression of ...