To investigate the effect of the calcium channel blocker verapamil on adipocyte differentiation and its mechanism of action. Preadipocytes from 3T3-L1 strain mouse embryos were cultured and differentiated into matured adipocytes in vitro. Verapamil was added to the culture medium in the concentration ...
图5 3T3-L1细胞成脂肪分化过程中不同时间点结果记录（最优条件下）注：文中图片来源于网络，仅供学习参考用 参考文献 [1] Ling HY, Wen GB, Feng SD, Tuo QH, Ou HS, Yao CH, Zhu BY, Gao ZP, Zhang L, Liao DF. MicroRNA-375 promotes 3T3-L1 adipocyte differentiation through modulation of extra...
(2018) Fucoidan Stimulates 3T3-L1 Adipocyte Differentiation by Modulating the Expression of PPARγ and C/EBPα. Marine Drugs 16(9): 344. Zhang Y, et al. (2018) The role of autophagy in 3T3-L1 adipogenesis. Acta Biochimica et Biophysica Sinica 50(6): 555-561....
Gao ZP, Zhang L, Liao DF. MicroRNA-375 promotes 3T3-L1 adipocyte differentiation through modulation of extracellular signal-regulated kinase signalling. Clin Exp Pharmacol Physiol. 2011 Apr;38(4):239-46. doi: 10.1111/j.1440-1681.2011.05493.x. PMID: 21291493; PMCID: PMC3086632. ...
小鼠胚胎成纤维细胞（前脂肪细胞）系（3T3-L1）是通过克隆分离得到的3T3(Swiss小白鼠)的连续亚株。当3T3-L1细胞从快速分裂到长满且接触抑制时，3T3-L1细胞经过前脂肪向脂肪样逆转。培养液中，高血清含量可以促进3T3-L1细胞内脂肪的积累。3T3-L1细胞系介绍 图1. 细胞系形态 3T3-L1细胞系应用 3T3-L1 细胞系不仅...
[1] Ling HY, Wen GB, Feng SD, Tuo QH, Ou HS, Yao CH, Zhu BY, Gao ZP, Zhang L, Liao DF. MicroRNA-375 promotes 3T3-L1 adipocyte differentiation through modulation of extracellular signal-regulated kinase signalling. Clin Exp Pharmacol Physiol. 2011 Apr;38(4):239-46. doi: 10.1111/j...
Adipocyte Differentiation Protocol: 1. Grow preadipocytes to confluency in 10% calf serum/DMEM 2. Two days post confluency (DAY 0) stimulate the cells with MDI induction media. You will notice a distinct change in the morphology of the cells (become more spindly) in the next 2 days. 3. ...
Adipocyte Differentiation Protocol: 1. Grow preadipocytes to confluency in 10% calf serum/DMEM 2. Two days post confluency (DAY 0) stimulate the cells with MDI induction media. You will notice a distinct change in the morphology of the cells (become more spindly) in the next 2 days. ...
Adipocyte differentiation of 3T3-L1 cells is routinely induced by a 2-day treatment of confluent preadipocytes with 1 μm dexamethasone and 0.5 mm MIX in the presence of fetal calf serum. 3–5 days after dexamethasone/MIX removal, the majority of cells attain an adipocyte phenotype. During ...
degradation and transcriptional activation are not always straight-forward. The E3 ligase SIAH2 has been shown to ubiquitinate PPARγ for degradation and to decrease its activity16. Depletion of SIAH2 in 3T3-L1 preadipocytes, however, prohibits adipocyte differentiation16. Another example is the E3 ...