The anagen-phase human hairs were plated on 3T3 cell feeder layers and were cultured in DAhEM, substituted with different' growth factors in a COincubator at 37℃. After 14 days in primary culture, 3T3 cells were removed with 0.02% EDTA and the keratinocytes were detached and disaggregated ...
The anagen-phase human hairs were plated on 3T3 cell feeder layers and were cultured in DAhEM, substituted with different' growth factors in a COincubator at 37℃. After 14 days in primary culture, 3T3 cells were removed with 0.02% EDTA and the keratinocytes were detached and disaggregated ...
Several methods were compared for their efficiency at supporting the growth of hybridoma cells at low cell densities. Soluble growth factors from different sources gave poor results, whereas actively metabolising syngeneic cells proved to be effective feeder systems. Of the latter, the transformed subli...
scriptional profiling for analysis of stem cell populationsJ . Genesis , 200337 (2) :57 - 63 . Lim JM , Bodnar A. Proteome analysis of conditioned medium from mouse embryonic fibroblast feeder layers which support the growth of human embryonic stem cellsJ . Protemomics , 2002 ,2 ...
5. Proteome analysis of conditioned medium from mouse embryonic fibroblast feeder layers which support the growth of human embryonic stem cells[J].Andrea Bodnar;Justin Wee Eng Lim,Proteomics.2002,第9期 6. RNA amplification and transcriptional profiling for analysis of stem cell populations[...
Establishment of a Hygromycin B Resistant NIH3T3 Cell Line as Feeder Layer; 具有潮霉素B抗性的NIH3T3细胞饲养层的制备 更多例句>> 5) NIH3T3 cell line NIH3T3细胞 1. Flourescence in situ hybridization analysis of the integration of mtDNA in the nuclear genes ofNIH3T3 cell line; ...
Influx of extracellular cADPR into CD38− 3T3 fibroblasts (23) or CD38low human hemopoietic progenitors (28) was recently demonstrated to occur upon transwell co-culturing either cADPR-responsive cell type over a feeder of CD38-transfected, cADPR-generating 3T3 fibroblasts. This influx causes a ...
CULTURE TECHNIQUE FOR HUMAN HAIR FOLLICLE KERATINOCYTES PRIMARY CULTURE AND SUBCULTURES ON 3T3 CELL FEEDER LAYERS XIA Long-Qing1, LEHMANN F2 PDF (PC) 407 赞 摘要/Abstract 摘要: 本文介绍一种人体毛囊外根鞘角阮细胞的培养技术。将拔取的生长期人发放在3T3细胞饲养层上和具有多种生长因子的DMEM中,在...
The 3T3 feeder cells in contact and the separate 3T3 feeder cell conditions yielded multilayered epithelial cell sheets 2–3 cells thick. The basal layers of these sheets had compact small cuboidal cells, while the suprabasal layer had large flat cells. In contrast without 3T3 feeder cell ...
(1982), Contact stimulated proliferation of cultured mouse epidermal cells by 3T3 feeder layers: inhibi- tion of proliferation by 12-O-tetradecanoyl-phorbol-13-acetate(TPA), J. Cell Physioi, 112, 76-82.Miller, D.R., Hamby, K.M., and Slaga, T.J. Contact- stimulated proliferation of ...