Gibco cell culture media & reagents Gibco growth factors Gibco FBS Countess II FL Automated Cell Counter MODIFY Lipofectamine transfection reagents Neon electroporation transfection system Invitrogen RNAi products Recombinant proteins Dynabeads cell separation technology ...
Gibco cell culture media & reagents Gibco growth factors Gibco FBS Countess II FL Automated Cell Counter MODIFY Lipofectamine transfection reagents Neon electroporation transfection system Invitrogen RNAi products Recombinant proteins Dynabeads cell separation technology ...
Prostaglandin Production by Normal and Transformed 3T3 Fibroblasts in Cell CultureProstaglandin E 2 and prostaglandin F 2伪 were quantitatively determined in culture media from Balb/c 3T3 fibroblasts and two virus-transformed derivatives of these cells. Regular and (simian virus 40)-transformed 3T3 ...
IR-3T3-L1 cells cultured for 96 h in the culture media containing 1 μmol ·L-1 DEX showed significantly increased glucose consumption (P=0.0003) as compared with the control group at 36 h (P<0.001). Conclusion 3T3-L1 cells can be induced into mature adipocytes by exposure to 1 μmol·...
Green H, Kehinde O. An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion. Cell. 1975 May;5(1):19-27. Green H, Kehinde O. Spontaneous heritable changes leading to increased adipose conversion in 3T3 cells. Cell. 1976 Jan;7(1)...
篇Cell media 细胞培养基 1001 Endothelial Cell Medium,500 ml 内皮细胞培养基1101 Smooth Muscle Cell Medium, 500 ml 平滑肌细胞培养基1201 Pericyte Medium, 500 ml 周边细胞培养基1521 Neuronal Medium, 500 ml 神经元细胞培养基1601 Oligodendrocyte Precursor Cell Medium, 500 ml 少突先驱胶质细胞培养...
The NIH 3T3 cells doubling time is 20-26 hours. Cell lines have a set of conditions for which they grow best. This includes a specific serum to provide additional growth factors, growth media, antibiotics to prevent bacterial growth and CO2 at a percentage for which the cells thrive (i.e...
Insulin Media (10mL/10cm plate; 5mL/6cm plate) To required volume of 10% FBS/DMEM add: 1:1000 Insulin METHOD Preadipocyte maintenance and passage: We plate the cells in 10% CS/DMEM on treated polystyrene culture dishes from Corning (Cat#430167) and incubate them at 37C in 10% CO2. It...
Briefly, cells were seeded at a density of 2 × 104 cells/well in 100 μL culture medium in 96-well microtiter plates. After incubation at 37°C and 5% CO2 for 48 h, media were removed from these cultured cells and added with MTT solution (5 mg/mL, 20 μL/well) for a 4-h ...
Insulin Media (10mL/10cm plate; 5mL/6cm plate) To required volume of 10% FBS/DMEM add: 1:1000 Insulin METHOD Preadipocyte maintenance and passage: We plate the cells in 10% CS/DMEM on treated polystyrene culture dishes from Corning (Cat#430167) and incubate them at 37C in 10% ...