for 20 min in propidium iodide (PI) staining buffer (10 lag/m1DNase-free RNase A, 50 μg/ml PI in PBS). The PT-stained cells were analyzed using BD Cellquest software on FAC-SCalibur™ Flow Cytometer (BD Biosciences, USA). eIF3m expression was found to be reduced (knockdown), as...
As used herein, the term “primer” refers to a short nucleic acid strand having a free 3′ hydroxyl group, which forms a base pair with a complementary template so as to serve as a starting point for the production of a new template strand. DNA synthesis or replication requires a suitabl...
Alternatively, the software could simply include a “check substrate” dialogue box or merely a reminder to use a particular printer tray that has been loaded with the appropriate substrate (e.g. tray 1 of the printer is always paper while tray 2 is always polymeric). As another alternative,...
Clinical studies and practice have shown that providing a negative pressure in proximity to a tissue site promotes the growth of new tissues at the tissue site. The application of negative pressure is successful in treating wounds. This treatment (frequently referred to in the medical community as ...