Capture(3C-qPCR) VuthyEa 1 ,ThierryForné 1 andFranckCourt 1,2 1 InstitutdeGénétiqueMoléculaire,UMR5535CNRSUniversityofMontpellier,France 2 Presentaddress:InsermUMR1103,CNRSUMR6293,F-63001Clermont-Ferrand,France andClermontUniversité,Universitéd'Auvergne,LaboratoireGReD,BP10448,F-63000 ...
Human LCE3C qPCR Primer Pair,即人LCE3C qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR),是一...
Quantitative PCRMany population-based methods investigating chromatin dynamics and organization in eukaryotes are based on the chromosome conformation capture (3C) method. Here, we provide an updated version of the quantitative 3C (3C-qPCR) protocol for improved and simplified quantitative analyses of ...
实时荧光定量PCR(quantitative real-time PCR,qPCR)是指在PCR反应体系中加入荧光基团,利用荧光信号积累实时监测整个PCR进程,*通过标准曲线对未知模板进行定量分析的方法。实时荧光定量PCR的化学原理包括探针类和非探针类两类,探针类是利用与靶细胞序列特异性杂交的探针来指示扩增产物的增加,非探针类是利用荧光染料或者特异...
实时荧光定量PCR(quantitative real-time PCR,qPCR)是指在PCR反应体系中加入荧光基团,利用荧光信号积累实时监测整个PCR进程,*通过标准曲线对未知模板进行定量分析的方法。实时荧光定量PCR的化学原理包括探针类和非探针类两类,探针类是利用与靶细胞序列特异性杂交的探针来指示扩增产物的增加,非探针类是利用荧光染料或者特异...
2×实时定量PCR扩增预混液 Hieff UNICON Advanced qPCR SYBR Master Mix ￥393.00 查看详情 一步法RT-qPCR试剂盒 SYBR绿色荧光定量试剂(One Step rt-qpcr Kit) ￥175.00 查看详情 DNA建库试剂盒(Hieff NGS® dna Library Prep Kit) ￥2755.00 查看详情 磁珠法土壤/粪便DNA提取试剂盒 MolPure® Mag Soil/St...
3C samples and controls were quantified using Qubit (Invitrogen), run on a 1% agarose gel and tested by qPCR with KAPA SYBR Fast (Sigma) to determine library quality. qPCR primers are in Supplementary Data 1. Only libraries with a digestion efficiency >70% were used for Capture-C. Librarie...
qPCR技术介绍(一) Real Time PCR,即实时荧光定量PCR,也被称为定量PCR或qPCR,是实时监测PCR扩增产物并进行解析的方法。 由于定量PCR具有操作简单、快速方便、灵敏度高、重复性好、污染率低等优点,被广泛应用于医学检测、药物疗效考核、基因表达研究、转基因研究、基因检测、...
To evaluate the extent to which the interaction with RBBP7 mediates the transcriptional regulatory function of LETR1, we performed a series of chromatin immunoprecipitation experiments followed by qPCR after LETR1-ASO2 knockdown (Fig.8e, f). First, we analyzed in LETR1-deficient samples the recru...
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