(1969) Metabolism of catecholamines-identification and quantification of 3- methoxy-4-hydroxyphenylglycol glucuronide in human urine. Biochemical Pharmacology, 18,1643-1654.Cohen, RA, Bridgers, WF, Axelrod, J, Weil-Malherbe, H, Labrosse, EH, Bunney, WE, Cardon, PV, Kety, SS (1962) The ...
Karoum F,Moyer-Schwing J,Potkin S G,et al.Presence of free,sulfate and glucuronide conjugated3-methoxy-4-hydroxyphenylglycol(MHPG)in human brain,cerebrospinal fluid and plasma. Brain Research . 1977Karoum F. , Moyer-Schwing J. , Potkin S. G. , and Wyatt R. J. ( 1977 ) Presence of ...
Peyrin, L, Pequignot, JM, Chauplannaz, G, Laurent, B, Aimard, G (1985) Sulfate and glucuronide conjugates of 3 methoxy- 4-hydroxy phenyl glycol (MHPG) in urine of depressed patients: central and peripheral influences. J Neural Transm 63: pp. 255-269...
Enzymatic hydrolysis of conjugated MHPG corroborated other work that plasma free MHPG, MHPG-glucuronide, and MHPG-sulfate were in roughly equal proportions. Unpredictably, in saliva, free MHPG was greater than 80% of the total. Salivary and plasma free HVA concentrations also correlated significantly,...
Free MHPG is determined directly, whereas MHPG sulphate and MHPG glucuronide are determined after enzymic hydrolysis. Since total MHPG, which represents the sum of these substances, is determined separately, a comparison of the results allows the effectiveness of the hydrolysis of the MHPG derivatives...
Conjugated (sulfate plus glucuronide) MHPG was adsorbed on a Sep-Pak Accell QMA cartridge and quantitatively eluted with 0.2 M NaCl. After enzymatic hydrolysis, deconjugated MHPG was extracted using a Sep-Pak Diol cartridge and analyzed in the same manner as unconjugated MHPG. The new method is...