The specific activity of the 3-ketolactose hydrolase is higher in extracts of strains belonging to biotype 2 than to biotype 1. The optimum pH of the enzyme in Tris-HCl buffer is 8.4–8.5. The Km value for 3-ketolactose is 2.2 × 102 M. The 3-ketolactose hydrolase is stimulated by ...
ChemInform Abstract: A 300 MH2 STUDY OF MELIBIOSE, LACTOSE AND 3-KETO-LACTOSE IN D2Omagnetic resonance, nuclear quadrupole resonance (organic substancescarbohydratesNo abstract is available for this article.doi:10.1002/chin.197539061DE BRUYN, A...
It was concluded that 3-ketolactose is not a necessary intermediate for the transport reaction of lactose to the cytoplasm and is on a sidetrack from the main catabolic route of lactose. Furthermore, in media with 2% lactose cell yield of 3-ketolactose-positive, but not of 3-ketolactose-...
A method to purify the enzyme which catalyses the oxydation of lactose to 4-0-/3-3-keto-o-galactoside-o-glucose, has been partially worked out and is the object of the present communication. This enzyme is most probably localized in the cytoplasm of the cells, since it has only been ...
Y. D. GaurH. MarečkováResearch Institutes for Crop ProductionFolia Microbiol (Praha)Folia MicrobiologicaGaur Y.D., Mareckova H. : Absence of 3-ketolactose production by Rhizobium phaseoli . Folia Microbiol. 22 , 311–312 (1977).
A.DeBruynM.AnteunisJ.vanBeeumenG.VerheggeBulletin des Sociétés Chimiques BelgesA 300 MHz study of melibiose,lactose and 3-ketolactose in D2O. De Bruyn A,Anteunis M,Van Beeumen J,et al. Bulletin des Sociétés Chimiques Belges . 1975...
NMR Experiments On Acetals. XIX. The NMR Analysis and Structure of 3-Keto-Lactose and β-d-Galactosedoi:10.1002/bscb.19690781109Wiley‐VCH Verlag GmbH & Co. KGaAM. AnteunisJ. van BeeumenA. De BruynJ. De Ley
A 300 MHz Study of Melibiose, Lactose and 3‐Keto‐Lactose in D2Odoi:10.1002/bscb.19750840501A. De BruynM. AnteunisJ. van BeeumenG. VerheggeWiley‐VCH Verlag GmbH & Co. KGaA
The lack of a free carbonyl function in 3-keto-lactose is due to the presence of the hemiacetal which is formed on crystallizing the compound from methanol. The hydroxyl group at the anomeric carbon atom has the β-configuration (1). It is suggested that this ketosugar prevails dominantly ...