[3] 3-HIB also increases fatty acid uptake and lipid accumulation in mouse skeletal muscle when administered in drinking water. The levels of 3-HIB in muscle are increased in db/db diabetic mice and in humans with diabetes. Human plasma levels are increased in obesity and correlate with a ...
Mutation analysis in the ALDH6A1 gene can reveal a cause of 3-hydroxyisobutyric aciduria, which may present with only slightly increased urinary levels of 3-hydroxyisobutyric acid, if a patient is metabolically stable.doi:10.1007/s10545-011-9381-xJrn Oliver Sass...
mRNA levels were normalized to the reference gene Rplp0 or 18S. 2.5. Muscle and adipose tissue gene expression analysis Total RNA was isolated from 100 mg frozen epididymal white adipose tissue (eWAT) and 30 mg frozen muscle tissue using RNA/DNA/Protein Purification Kit (Norgen Biotek Corp.)...
To date, only two bacterial TSRs have been characterized at the molecular and biochemical levels even though related sequences can be found in the genomes of archaea, fungi, plants and animals [4]. ...
Application of the method to measure S-3-hydroxyisobutyrate in plasma obtained from normal, 48-h starved, and mildly and severely diabetic rats gave levels of 28, 42, 112, and 155 渭 m, respectively.doi:10.1016/0003-2697(90)90687-5Paul M. Rougraff...
Relative mRNA levels were: kidney greater than liver = heart greater than muscle. The amino acid sequence of 3-hydroxyisobutyrate dehydrogenase shows similarity to several other pyridine nucleotide-dependent dehydrogenases. The resemblance to malate and lactate dehydrogenases suggests that the nucleotide-...