Method 2 is more convenient to use but it depends on having previously purified phosphohexuloisomerase as a coupling enzyme. The purification procedure involves preparation of cell-free extract, preparation of particulate fraction, solubilization with l M NaCl, ammonium sulfate fractionation, diethyl...
The formaldehyde-fixing enzymes, 3-Hexulose-6-phosphate synthase (HPS) and 6-phospho-3-hexuloisomerase (PHI), are the key enzymes catalyzing sequential reactions in the ribulose monophosphate (RuMP) pathway. In this study, we generated two fused gene constructs of the hps and phi genes (i....
The two key enzymes of the ribulose monophosphate pathway, D-arabino-3-hexulose 6-phosphate synthase (hps1A) and phosphohexuloisomerase (phi) were found probably in operon, however, three other hps-like genes (hps1B, hps2A, and hps2B) were identified independently. The one-carbon unit (...
Orita I; Sakamoto N; Kato N;.Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho- 3-hexuloisomerase.Applied Microbiology and Biotechnology.2007.439-445Orita I,Sakamoto N,Kato N. Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexulo...
( 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase activities are expressed constitutively in archea [13]) [1,3,4,8,13,14,22]3-hexulose-phosphate synthase [18]3-hexulosephosphate synthase [5,6,12,20,21] d -arabino-3-hexulose 6-phosphate formaldehyde lyase [2,12] d ...
Class 3.4–6 Hydrolases, Lyases, Isomerases, Ligases Dietmar Schomburg& Ida Schomburg Part of the book series:Springer Handbook of Enzymes((HDBKENZYMES,volume 10)) 1582Accesses Nomenclature EC number 4.1.2.43 Systematic name d-arabino-hex-3-ulose-6-phosphate formaldehyde-lyase (D-ribulose-...