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TG100-115 was tested in a phase I/II clinical trial in patients with STEMI in 2005, although results have not been published to date. Despite the proinflammatory effects of PI3Kγ in leukocytes, this isoform in cardiomyocytes contributes to normal contractility (120), independent of the kinase ...
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5′-AGCTTTTCCAAAAACCTAAGGTTAAGTCGCCCTTCTCTTGAAAGGGCGACTTAACCTTAGGGG-3’. Western blotting and antibodies Western blot analysis was performed as previously described31. Briefly, cell lysates were made in mRIPA buffer (containing 150 mmol/L NaCl, 0.1% SDS, 1% Nonidet P40, 1% sodium deoxycholate ...
SRR2058107.412129 0 10_w_c 42386003 1 90M * 0 0 TGGATTGGAAGGTAATTATTATTGAATGGAATTGAATGGAATTATTGAATGGATTTGAATGGAATAATTATTGAATGGAATTGAATGGAA IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII PG:Z:SNAP NM:i:3 RG:Z:FASTQ PL:Z:Illumina PU:Z:pu LB:...
Then, qPCR primers targeting GAPDH (forward: 5'-GAAGGTGAAGGTCGGAGTC-3' and reverse: 5'-GAAGATGGTGATGGGATTTC-3'), viral genome (forward: 5'-CGAAAGGTAAGATGGAGAGCC-3' and reverse: 5'-TGTTGACGTGCCTCTGATAAG-3') were used. 2.13. Statistical analysis All experiments were repeated, and ...
(1 × 106cells/well in a 6 well plate) as previously reported34,47. The relative mRNA expression was calculated and expressed as 2−(ΔΔCt). Forward and reverse primer sequences were the following: mouse GAPDH, ctgagtatgtcgtggagtctac and gttggtggtgcaggatgcattg; mouse Pro-...