Jiye Shi, Weiliang Zhu, D3Pockets: A Method and Web Server for Systematic Analysis of Protein Pocket Dynamics.J. Chem. Inf. Model.2019, 59, 8, 3353–3358. DOI:https://doi.org/10.1021/acs.jcim.9b00332
3Below: Tales of Arcadia: Created by Guillermo del Toro. With Tatiana Maslany, Diego Luna, Nick Offerman, Frank Welker. A pair of teenage royals and their bodyguard escape from their home planet and try to blend in on Earth.
After washing three times in 1 × PBS for 10 min, fixed neurons were incubated with primary antibodies diluted in 1 × GDB (0.1% gelatin, 0.3% Triton X-100, 0.45 M NaCl, 17.7 mM sodium phosphate buffer, pH 7.4) in a humidified chamber overnight at 4 °C. Primary antibodies...
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Cells were washed three times with PBS and lysed in ice cold extraction buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1% NP, 0.1% SDS, and 1× protease inhibitor cocktail). Whole cell lysates were centrifuged at 12000 g at 4 °C. Soluble fractions were mixed with 5× ...
(Life Technologies) for 2 hours at room temperature in the dark. Then sections were washed several times in PBS and mounted with VectaShield (Vector Laboratories, Burlingame, CA) containing Hoechst (Life Technologies). For each antibody, control IHC was carried out by omitting the primary ...
Homozygotes of the ApoEε4 allele have 15 times more risk of developing AD than a noncarrier of this allele (10), and each copy of the allele lowers the age at onset by 10 y (9). Despite the large amount of research in AD over the past 3 decades, the exact disease-related ...
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(DC) pulses), and the stimulus intensity was then set at three times this threshold for the rest of the experiment. A common stimulation sequence was programmed using the Spike2® programming language. The stimulation sequence began with and ended with a series of 12 single shocks delivered ...
Slides were washed once more in 1X PBS for three times and then mounted in 1 in 5000 DAPI diluted in 1:1 glycerol:1X PBS. Glycerol:PBS mounting agent can be easily removed with the coverslip to perform sequential immuno-staining. Because serial-section ribbons occupy most of the slide, ...