To thoroughly characterize the details of the PcaD active site, we sought to obtain a co-crystal structure with the substrate analog 4-hydroxy-3-pentenoic acid γ-lactone (Fig. 3a), which differs from the native substrate 3-oxoadipate-enol-lactone in that it lacks a terminal carboxylate grou...
The catechol and protocatechuate branches of the 3-oxoadipate pathway, which are important for the bacterial degradation of aromatic compounds, converge at the common intermediate 3-oxoadipate enol-lactone. A 3-oxoadipate enol-lactone-hydrolyzing enzyme, purified from benzoate-grown cells of Rhodococc...