PcaD (3-oxoadipate-enol-lactonase) is an essential enzyme that catalyzes the conversion of 3-oxoadipate-enol-lactone into β-ketoadipate at the junction of the aromatic degrading protocatechuate and catechol pathways. The high-resolution, product analog (levulinic acid) bound structure of PcaD ...
The catechol and protocatechuate branches of the 3-oxoadipate pathway, which are important for the bacterial degradation of aromatic compounds, converge at the common intermediate 3-oxoadipate enol-lactone. A 3-oxoadipate enol-lactone-hydrolyzing enzyme, purified from benzoate-grown cells of Rhodococc...