中文名称:3'-O-氨基-2'脱氧鸟苷-5'-三磷酸 英文名称:3'-ONH2-dGTP CAS:1220515-98-9 纯度规格: >=98% 产品类别: Nucleotides 级别: 医药级 货号: dNTP012 Deoxy-ribonucleoside triphosphate(脱氧核糖核苷三磷酸,dNTP)作为PCR反应的原料在体系中不可或缺,dNTP包括dATP,dGTP,dCTP,dTTP。 dNTP的质量及浓度...
C₁₀H₁₉N₄O₁₃P₃.xNa作⽤靶点:BiochemicalAssayReagents;DNA/RNASynthesis作⽤通路:Others;CellCycle/DNADamage储存⽅式:PleasestoretheproductundertherecommendedconditionsintheCertificateofAnalysis.BIOLOGICALACTIVITY⽣物活性3'-ONH2-dCTPsodium带标记修饰的脱氧寡核苷酸(dNTP),能够释放焦磷酸...
产品描述 3'-ONH2-dTTP sodium 是带标记修饰的脱氧寡核苷酸 (dNTP),能够释放焦磷酸产生荧光,在基因合成与测序中具有特殊应用。更多详情参考:https://www.medchemexpress.cn/3-onh2-dttp-sodium.html上一篇:[Leu5]-Enkephalin TFA 下一篇:Luteoreticulin ...
In a 50-L reaction, the indicated amounts of each primer and 100 ng of genomic DNA were added to a mixture containing a final concentration of dNTP, each 12.5 M; 35S-dATP and 35S- dCTP (Amersham Pharmacia Biotech, Baie d'Urfe´, Que´bec, Canada), 12.5 Ci each; Taq...
First-strand cDNA was synthesized from 1 µg of total RNA, using 0.5 μg of Oligo(dT) 12–18 prim- ers (Invitrogen, Carlsbad, CA, USA), 250 µM of each dNTP and 200U of RevertAid reverse transcriptase ®(Fermentas, Vilnius, Lithuania). Real-time PCR was performed using...
Briefly, PCR components included 0.4 µM of primer, 0.2 mM dNTP mix, 1.5 mM MgCl2, 10X PCR buffer, and 0.1 µl Platinum Taq DNA polymerase, and 2.5 μl of cDNA in a total volume of 25 µl. PCR was performed under the following conditions: initial denaturation at 94 °C for 5...
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One microgram (1 mg) of total RNA was reverse transcribed using Super-Script III Reverse Transcriptase (Invitrogen), random pdN6 primers (Roche), 0.1 mM DTT (Invitrogen) and 10 mM dNTP (Invitrogen) in a 20 ml volume for 90 min at 50 °C on a Veriti 96-well thermocycler (Applied ...
In addition, the d-spacing of each crystal orientation observed in the XRD pattern is also calculated based on the diffraction angle using Bragg's law, the result obtained matches closely with those calculated with the SMbAynElTOinDh2ee-paCcraaNtsttwaTelrseynehtipfycrbvoaromciltdtiavtchmiatetym...