The first committed step in VLCFA biosynthesis is condensation of C 2 units to an acyl CoA by 3-ketoacyl CoA synthase (KCS). In this study, two KCS genes, KCS20 and KCS2 / DAISY , that showed higher expression in stem epidermal peels than in stems were isolated. The relative ...
The 3-ketoacyl-ACP synthase (KAS) III proteins are one of the most abundant enzymes in nature, as they are involved in the biosynthesis of fatty acids and natural products. KAS III enzymes catalyse a carbon–carbon bond formation reaction that involves the α-carbon of a thioester and the ...
超长链脂肪酸(very-long-chain fatty acids,VLCFAs)及其衍生物是花粉包被的重要组成部分,3-酮脂酰辅酶A合成酶(3-ketoacyl-CoA synthase,KCS)是VLCFAs合成的关键限速酶.为了深入了解脂类在花药中的合成,及脂类在花粉发育和受精过程中功能,我们系统地分析了KCS家族基因在花药发育过程中的表达模式.我们构建了KCSs-...
3-ketoacyl-CoA synthase (KCS) gene Nervonic acid is a Very Long-Chain Monounsaturated Fatty Acid (VLCMFA), 24:1 螖15 ( cis -tetracos-15-enoic acid) found in the seed oils of Lunaria annua , ... Y Guo,E Mietkiewska,T Francis,... - 《Plant Molecular Biology》 被引量: 67发表:...
Ghanevati, “Engineering and Mechanistic Studies of Fatty Acid Elongase1 β-Ketoacyl-CoA Synthase, FAE1 KCS,” A Dissertation, submitted to the Faculty of Miami University, Oxford, Ohio, 2000. Han, “β-Ketoacyl-CoA Synthase Gene from Brassica napus L.: Functional Characterization and Promote...
This finding suggested that the elongation of 16:1 A4-ACP and 16:0-ACP in coriander seed is catalyzed by a 3-ketoacyl-ACP synthase (KAS) 1-type enzyme(s), rather than a KAS II-type activity that is typically associated with 16:0-ACP elongation. Consistent with this, a cDNA for a ...
1.FabB(β-ketoacyl-acyl carrier protein synthaseⅠ)and FabF(β-ketoacyl-acyl carrier protein synthaseⅡ)are two key enzymes of fatty acid biosynthesis in E.体内体外研究显示:fabF1基因能互补大肠杆菌fabB突变,FabF1具有β酮脂酰ACP合成酶Ⅰ(FabB)活性;fabF2能互补大肠杆菌fabF突变,FabF2具有β酮脂酰...
脂肪酰-CoA 延长酶属于膜结合的多酶复合体, 包括β-酮脂酰-CoA 合酶(3-ketoacyl-CoA synthase,KCS)、β-酮脂酰-CoA 还原酶(3-ketoacyl-CoA reductase, KCR)、β-羟脂酰-CoA 脱水酶(3-hydroxacyl-CoA dehydratase, HCD)和反 式烯脂酰-CoA 还原酶(trans-2,3-enoyl-CoA reductase, ECR), 分别催化...
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The first step of VLCFA synthesis is mediated by 3-ketoacyl-CoA synthase (KCS), which catalyzes the condensation of a C2 unit from malonyl-CoA to acyl-CoA. In the present study, we investigated the role of KCS4 in pollen tube and root growth. KCS4 was predominantly expressed in shoot ...