3-Ketoacyl-ACP synthases IIIFatty acid biosynthesisBranched-chain DSF-family signalsPathogenesisThe 3-ketoacyl-ACP synthase III (FabH), a key enzyme for bacteria growth, catalyses the last step of the initiation of bacterial fatty acid synthesis. Rice bacterial blight is caused by the pathogen...
专利名称：3-ketoacyl-ACP-reductase (FabG) of Staphylococcus aureus 发明人：Jianzhong Huang,Damien McDevitt 申请号：US09/238481 申请日：19990128 公开号：US06110704A 公开日：20000829 专利内容由知识产权出版社提供 摘要：The invention provides fabG polypeptides and polynucleotides encoding fabG polypeptides ...
Efforts to understand the complex biology of A-encoded ketoacyl reductase mutants, a sub-population of E. coli BL21(DE3) host cells was selected from a small-opaque colony. This empirically selected host, named BL21(DE3)NH, allowed stable expression of these mutant proteins. Although the ...
3-酮基脂酰ACP还原酶催化3-酮基脂酰ACP还原为3-羟基脂酰ACP,是细菌脂肪酸合成反应的关键酶之一.为了明确该酶中110位的保守天冬酰胺残基在酶催化活性和酶结构中的作用,本研究采用基因定点突变和蛋白质表达纯化技术,获得了大肠杆菌3-酮基脂酰ACP还原酶FabG的两个突变蛋白:FabG N110Q和FabG N110L.圆二色谱结果显...
3-ketoacyl-acyl-carrier protein synthase I (KASI) plays crucial roles in tobacco 来自 学术范 喜欢 0 阅读量: 124 作者:Yang, Tianquan,Xu, Ronghua,Chen, Jianghua,Liu, Aizhong 摘要: 中国科学院机构知识库(CAS IR GRID)以发展机构知识能力和知识管理能力为目标,快速实现对本机构知识资产的收集,长期...
ketoacyl-thioesters are unstable, the reactions contained the purifiedE. colifatty-acid synthetic enzymes; 3-ketoacyl-ACP reductase (FabG), 3-hydroxyacyl ACP dehydratase (FabA), and enoyl-ACP reductase (FabI) and NADH to convert the condensation product to the stable fully saturated form. By ...
1.FabB(β-ketoacyl-acyl carrier protein synthaseⅠ)and FabF(β-ketoacyl-acyl carrier protein synthaseⅡ)are two key enzymes of fatty acid biosynthesis in E.体内体外研究显示:fabF1基因能互补大肠杆菌fabB突变,FabF1具有β酮脂酰ACP合成酶Ⅰ(FabB)活性;fabF2能互补大肠杆菌fabF突变,FabF2具有β酮脂酰...
Structure of 3‐oxoacyl‐(acyl‐carrier protein) synthase II from Thermus thermophilus HB8 The β-ketoacyl-(acyl carrier protein) synthases (β-keto-ACP synthases; KAS) catalyse the addition of two-carbon units to the growing acyl c... B Bagautdinov,Y Ukita,M Miyano,... - 《Acta ...
3-ketoacyl moieties are also referred to as β-ketoacyl moieties. Each elongation cycle involves three additional enzymatic steps in addition to the condensation reaction discussed above. Briefly, the 3-ketoacyl condensation product is reduced to 3-hydroxyacyl-ACP, dehydrated to the enoyl-ACP, and...
3-Oxoacyl-[ACP] reductase (E.C. 1.1.1.100, alternatively known as beta-ketoacyl-[ACP] reductase), a component of fatty acid synthetase has been purified from seeds of rape by ammonium sulphate fractionation, Procion Red H-E3B chromatography, FPLC gel filtration and high performance hydroxyapatite...