Following fixation, coverslips were washed with PBS prior to blocking with 3% BSA in PBS at room temperature for 1 h. Cells were stained with indicated antibodies (see Supplementary Table 3) for either 1 h at room temperature or overnight at 4 °C. Coverslips were washed with PBS ...
放置磁力架上面,去除上清。磁珠用三羟甲基氨基甲烷/吐温缓冲液(TBS/T)清洗2遍,用40 μL 2倍SDS-PAGE上样缓冲液将蛋白A/G磁珠-抗原抗体复合物重悬混匀,Western印迹进行特异性抗体鉴定。5.线粒体活性氧簇(ROS)检测:5×105Nthy-ori...
we examined brucine-induced changes in H2O2and the role of H2O2in regulation of iron increase. As shown in Fig.4a, brucine improved intracellular H2O2in a time-and dosage-dependent manner, which was paralleled by depletion of intracellular antioxidant GSH at each indicated dosage or incubation time...
实验证明,这种非特异性染色主要是酶标抗体/桥抗体与组织γ-球蛋白结合引起的,用封闭性阻断剂及正常血清等分别孵育切片,并在稀释抗体时,加入适量的BSA,可以降低此类非特异性结合。BSA、封闭性阻断剂与IgG(正常血清)的等电点不同,重叠应用,阻断效果尤佳。另外,铺片的细胞膜完整,不利于抗体的穿透,特别是PAP复合物等...
TBS, pH 7.0 to 8.0, with 0.1% BSA 内含物 0.09% sodium azide 保存条件 4° C 运输条件 Ambient (domestic); Wet ice (international) 产品详细信息 The recommended shelf life for this product is 1 year from date of receipt. Based on 100% sequence identity, this antibody is predicted to react...
1. 完成转膜后,将转印膜放入到杂交孵育盒中,加入 10-20 ml 1× Rapid Blocking Buffer (TBS-T),覆盖过载体表面,置于桌面水平摇床室温孵育 10 min 左右; 注:本产品封闭 10 min 优于常规 BSA 封闭 1 h,对于背景较高的抗体,可尝试将封闭时间延长至 30~60 min。 2. 封闭后的膜即可用于一抗孵育等后续实验...
储存液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When ...
储存液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 别 名:Activating transcription factor 3; ATF3deltaZip2; ATF3deltaZip2c; ATF3deltaZip3; cAMP dependent transcription factor ATF3; Cyclic AMP dependent transcription factor ATF3; Cyclic AMP-dependent transcription fact...
[IF=5.154]Wu et al. Roles of Pannexin-1 Channels in Inflammatory Response through the TLRs/NF-Kappa B Signaling Pathway Following Experimental Subarachnoid Hemorrhage in Rats. (2017) Front.Mol.Neurosc. 10:175WB ; Rat. PubMed:28634441
储存液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. PubMedPubMed 产品介绍Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in ...