Acquiring ground state pluripotency: switching mouse embryonic stem cells from serum/LIF medium to 2i/LIF medium. Methods Mol Biol 2016;1341:41–8.Tosolini M, Jouneau A. Acquiring ground state pluripotency: switching mouse embryonic stem cells from serum/LIF medium to 2i/LIF medium. Methods ...
Mouse ES cells27 were maintained under feeder-free conditions in LIF-2i medium on 0.1% gelatin-coated culture plates28. The cells were trypsinized, washed, counted and assessed for cell viability. Animals Male and female wild type CD-1 mice (Charles River) between postnatal days 21–37 were...
Conversion of partial- or pre-iPS cells into fully pluripotent cells in the presence of LIF Components 1 bottle (200 ml) of Cellartis mES/iPSC Culture Basal Medium 1 tube (200 µl) Cellartis 2i mES/iPSC Supplement References Malaver-Ortega, L. F. et ...
By company size Enterprises Small and medium teams Startups Nonprofits By use case DevSecOps DevOps CI/CD View all use cases By industry Healthcare Financial services Manufacturing Government View all industries View all solutions Resources Topics AI DevOps Security Software Development...
Lif study of the I2+F --> IF+I reaction: population of the v' = 0 level of IF 来自 Semantic Scholar 喜欢 0 阅读量: 33 作者:B. Girard,N. Billy,G. Gouedard,J. Vigué 摘要: We have attempted to detect the v' =0 level in IF molecules produced by the reactive collision I2+F...
新目标大学英语(第二版)视听说教程第2册 徐锦芳课后习题答案 新 目标大学英语 (第二版 )视 听说教程第2册徐锦芳课后习题答案 PoSSible AnSWerS / 1∙ Read the following quotes. DiSCUSS in PairS and Share With each Other your UnderStanding Of these quotes. The four quotes highlight the beauty and ...
1 The abbreviations used are: TGF-β, transforming growth factor-β; IL, interleukin; OSM, oncostatin M; DMEM, Dulbecco's modified Eagle's medium; FCS, fetal calf serum; BSA, bovine serum albumin; DMS, dimethyl sulfate; bp, base pair(s); TNF-α, tumor necrosis factor-α. ...
The 2i medium boosted mRNA abundances of Pouf5, Sox2, Nanog, GFRα‐1, PLZF, anti‐apoptosis gene Bcl2, LIF receptor gene LIFR and enhanced PLZF protein expression, but suppressed mRNA expressions of spermatogonial differentiation marker c‐kit and pro‐apoptotic gene Bax, in the cultured ...
A more recent culturing system with two inhibitors (for GSK3尾 (CHIR99021) and MEK1/2 (PD0325901)) and LIF enables the derivation of mESC lines from various mouse strains. Here we describe methods for the mESC growth and maintenance in each medium composition as well as their adaptation to...