(2)我若用2-delta delta ct法做相对定量时可以不管扩增效率,不要作标准曲线,直接在相对定量模式下将内参和目的基因相应的样品放置同一板上跑即可,然后得到的数据用2-delta delta ct法计算即可,是吗 (3)我若用双标准曲线法(内参和目的基因各10倍梯度稀释分别作标曲),则将标曲的样品和其他检测的样品一起在绝对...
想用2-delta delta ct法做相对定量，但是前提是扩增效率在80%-120%之间，ABIM7300相对定量模式下不能...
1.The boundary element to inverse 3-D density interface,in fact,is that of the surface in-tegal,it constructs a linear equation system to solve the interface tomography by scattering the surface of density inhomogeneities,with theCT algorithm,it is greatly to save memory space and operation time...