In the areas of toxicology and anticancer drug development it concerns a significant difference of responses to proapoptotic factors of the cells cultured in 2D versus 3D environment. Besides, the clear-cut differences have been found in cell polarity, cytoskeleton structure, distribution of receptors...
最近的发展明确表明,对于工业应用而言,从2D过渡到3D细胞培养是有前途的,但技术的成熟度和成本仍然是实现这一转变的主要关切点。仍然需要大量努力来确保可重复性、高通量分析、兼容的读数技术和自动化,以建立标准化和验证的3D细胞培养模型。 参考文献: Edmondson, Rasheena et al. “Three-dimensional cell culture sy...
Cell culture on a chip;On-chip cell culture Definition Cell culture on chip is defined as those methods to cultivate biological living cells on two- and three-dimensional (2D and 3D) chip devices. Microchip techniques can provide many advantages for cell culture systems because the scale of the...
[2] Ravi M , Paramesh V , Kaviya S R , et al. 3D Cell Culture Systems: Advantages and Applications[J]. Journal of Cellular Physiology, 2015, 230(1):5-26. [3]Koledova, Zuzana. 3D Cell Culture: An Introduction[J]. Methods in Molecular Biology, 2017, 1612(Chapter 1):1. 每周五下...
5. Tibbitt MW, Anseth KS. 2009. Hydrogels as extracellular matrix mimics for 3D cell culture. Biotechnol. Bioeng.. 103(4):655-663. http://dx.doi.org/10.1002/bit.22361 6. Lin C, Anseth KS. 2009. PEG Hydrogels for the Controlled Release of Biomolecules in Regenerative Medicine. Pharm Re...
However, at the highest trehalose concentration (1200mM) and in the absence of the pre-incubation step, there was no statistical difference at the 95% CI (p=0.0212) between the viability of the HEK cells under 2D and 3D culture conditions estimated to be 17.9 卤 4.6% and 14.0卤3.6%, ...
Tools for 2D and 3D Neuronal Cell Culture Improve Image Quality in 2D and 3D Biological Samples Specificity Analysis of Antibodies That Recognize Histone Posttranslational Modifications Time-Saving Immunoassay Tools M...
Here, to acquire more precise information about plasma-tumor interactions, the plasma-induced cytotoxicity was tested in both 2D cell culture and 3D tumor-spheroid culture. The results indicated that the toxic effects were markedly reduced in 3D cancer cells compared with 2D cells. We found that ...
However, the inhibitory effect of TGF-β on adipogenesis decreased under 3D spheroid culture conditions and TGF-β isoforms did not affect adipogenesis-induced (a) enlargement and downsizing of 3T3-L1 spheroids, (b) increase in BODIPY lipid staining intensity, and (c) up-regulation of the mRNA...
Cancer Cell Int (2019) 19:16 https://doi.org/10.1186/s12935-019-0733-1 Cancer Cell International PRIMARY RESEARCH Open Access The effect of 2D and 3D cell cultures on treatment response, EMT profile and stem cell features in head and neck cancer Styliani Melissaridou1†,...