因此我们认为HEK293细胞或许更适合进行KO、KI或点突变等基因编辑实验。而293T细胞，由于转入了SV40T-antigen基因，使得包含SV40ori的质粒能够在该细胞系中显著扩增，从而促进基因的表达。加上转入SV40大T抗原后，细胞增殖速度较HEK293有明显提升，因此在293T细胞上更适合做质粒转染、病毒包装等方面的实验。总之，293系列...
在其它物种,尤其是仓鼠],SV40引起多种肿瘤,通常是肉瘤。在大鼠中,这种致瘤的SV40大T抗原被用来建立关于原始神经外胚层瘤和成神经管细胞瘤的脑肿瘤模型 SV40 T antigen SV40大T抗原(猿猴空泡病毒40TAg)是六聚体蛋白,它是衍生自SV40病毒的显性作用癌蛋白。TAg能够诱导多种细胞类型的恶性转化。TAg的转化活性很大程度...
HEK293 cells are immortalized byAd 5 E1A and E1B. 293 cells have highly aberrant karyotype and constitutively express Ad5 E1A/E1B proteins, which deregulate pRB/p53pathways. HEK293T cells also expressSV40 large T antigen.Thus, the cells lack normal pRb and p53 functions. The tumorigenicity of...
as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to...
Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes ...
Next-generation sequencing (NGS) of SV40 T antigen-negative cell clones, as well as the parental HEK293T cell line, confirmed the presence of deletions within the SV40 large T antigen coding sequence. In addition, targeted locus amplification (TLA) sequencing9,10 allowed further characterization ...
HEK293T是源自HEK293原始细胞系的子代细胞系,转染携带SV40复制起点的质粒载体,产生大量重组蛋白。293T细胞表达 E1A蛋白,S40 large T抗原,含有S40复制起始点与启动子区的质粒可以复制。293T细胞非常容易转染,用磷酸钙、PEI或脂质体转染,效率均可达90%以上(你的转染效率又有多高呢?doge)。
The AAVpro 293T Cell Line is a subclone of the transformed human embryonic kidney cell line HEK 293, which is highly transfectable and supports high levels of viral protein expression. The cell line also constitutively expresses the simian virus 40 (SV40) large T antigen. When combined with ...
Lentivirus production is influenced by SV40 large T-antigen and chromosomal integration of the vector in HEK293 cells. Currently, lentiviral vectors for research and gene therapy are produced from 293-T cells that are transiently transfected with plasmids encoding the vecto... L Gama-Norton,L Botez...
293T cells are 293 cells transformed by expression of the large T antigen from SV40 virus that inactivates pRb