They have developed an RNA fluorescence in situ hybridization (FISH) method for high-sensitivity detection of SARS-CoV-2 mRNAs in HEK 293T cell cultures as a model. After transfection of HEK 293T cells with plasmids, Spike (S)/envelope (E) proteins and their mRNAs were clearly detected insi...
[2] Qin, Xu-Hui, et al. "Knockout of the placenta specific 8 gene affects the proliferation and migration of human embryonic kidney 293T cell." Cell biochemistry and biophysics 78.1 (2020): 55-64.[3] Lo, Chiu-An, Alexander W. Greben, and Brian Edwin Chen. "Generating stable cell ...
Methods: To better investigate the functions and mechanisms of human miR-210, therefore, we derived the human miR-210 gene knockout (KO) 293T cell lines using the CRISPR/Cas9 technology. We then examined the cellular phenotypes and gene expression profiles of 293T cel...
细胞:Primary CRC cell lines (SW480, HT-29, and HCT-116), metastatic CRC cell line SW620, NCM460 normal colon mucosal epithelial cell line, and 293T cell line2022年影响因子/JCR分区:4.501/Q2 Low-dose metformin targets the lysosomal AMPK pathway through PEN2. Nature. 2022 Mar;603(7899)...
ApplicationCell Culture / Growth Conditions,Protein Expression,RNAi,Stable Cell Transfection,Transfection: Gene Expression,Transfection: Gene Silencing,Transient Transfection Products Catalog #NameSizePrice (ARS)Qty 0835072IMCustom Media: GENTAMICIN1 each ...
If cells are cultured in medium containing FBS that is not reduced in tetracycline, there may be low basal expression of the gene of interest in the absence of added tetracycline. In such cases, we recommend purchasing tetracycline-reduced FBS from our Gibco Cell Culture Division. To be ...
[2] Qin, Xu-Hui, et al. "Knockout of the placenta specific 8 gene affects the proliferation and migration of human embryonic kidney 293T cell." Cell biochemistry and biophysics 78.1 (2020): 55-64. [3] Lo, Chiu-An, Alexander W. Greben, and Brian Edwin Chen. "Generating stable cell ...
Derivation The 293T cell line, originally referred as 293tsA1609neo, is a highly transfectable derivative of human embryonic kidney 293 cells, and contains the SV40 T-antigen. Clinical Data - Antigen Expression - Receptor Expression - Oncogene - Genes Expressed - Gene expression databases...
Cell lysates were harvested at indicated times followed by immunoblot analysis with anti-RIG-I and anti-β-actin antibodies. The results showed that the expression of RIG-I could not be detected in IBV or SeV infected...
[2] Qin, Xu-Hui, et al. "Knockout of the placenta specific 8 gene affects the proliferation and migration of human embryonic kidney 293T cell."Cell biochemistry and biophysics78.1 (2020): 55-64. [3] Lo, Chiu-An, Alexander W. Greben, and Brian Edwin Chen. "Generating stable cell line...