Serum-free treated NIH/3T3 cells and 10 % fetal bovine serum treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29018-1-AP (Phospho-YAP1 (Ser397) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-bl...