WB analysis using 28961-1-AP Non-treated HT-29 and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28961-1-AP (Phospho-P53(Ser15) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antib...