The ubiquitin–26S proteasome system at the nexus of plant biologyRichard D. Vierstra Nature Reviews Molecular Cell Biology volume 10, pages 385–397 (2009)Cite this article 11k Accesses 1037 Citations 3 Altmet
一篇最近发在Nature Chemical Biology的文章,标题是Targeted degradation via direct 26S proteasome recruitment。 蛋白降解靶向嵌合体(proteolysis-targeting chimera, PROTAC),是由Craig Crews等(2001)提出的概念,并且他们当年的文章也可以算得上是概念验证和开山之作。他们开发了一种分子叫做Protac-1,是由卵假散囊菌...
Conclusions: The findings of this study demonstrate that genetic blockade of p-S14-RPN6 exacerbates tauopathy pathology in both the brain and heart. This is the first to have established the role of p-S14-RPN6 and, by extension, the significance of PKA-mediated proteasome activation in AD/...
In eukaryotes, this is achieved by the tagging of proteins with ubiquitin and their subsequent recognition and degradation by the 26S proteasome. In plants, genetic analysis has identified many genes that regulate developmental pathways. Subsequent analysis of these genes has implicated ubiquitin and ...
Both mitochondrial mutations in respiratory complex I and treatment with the anti-diabetic drug metformin impair 26S proteasome activity. Defective 26S assembly is reversible and can be overcome by supplementation of aspartate or pyruvate. This metabolic regulation of 26S activity involves specific ...
Here, we describe a new strategy of targeted protein degradation through direct substrate recruitment to the 26S proteasome. The proteolytic complex is essential and abundantly expressed in all cells; however, proteasomal ligands remain scarce. We identify potent peptidic macrocycles that bind directly ...
resulting in multiple types of proteasome complexes that co-exist in cells. The 19S RP has been widely studied and together with 20S CP forms the best known 26S proteasome, a 2.0–2.5 MDa machinery that degrades the vast majority of poly-ubiquitinated as well as some non-ubiquitinated protei...
BMC Plant Biol (2021) 21:486 https://doi.org/10.1186/s12870-021-03234-9 RESEARCH Open Access Oxidative and salt stresses alter the 26S proteasome holoenzyme and associated protein profiles in Arabidopsis thaliana Diana Bonea1,2, Jenan Noureddine1,2, Sonia Gazzarrini1,2* ...
The specificity of the ubiquitin (Ub) isopeptidase in the PA700 regulatory complex of the bovine 26 S proteasome was investigated. Disassembly of poly-Ub by this enzyme is restricted to the distal-end Ub of the substrate, i.e. the Ub farthest from the site of protein attachment in poly-...
Another interesting feature revealed by our structural and biochemical studies is that ATP is not required for the interaction between S6 ATPase and gankyrin, at least at the nonproteasome level. As none of the structures of the six ATPases of the 19S regulatory particles is known, we ...