These are ATPase subunits as well as non- ATPase subunits and seem specific for a cell line. A down- regulation of 19S sub-units could sustain the global de- creased proteasome activity observed in REC1 cells an
The 26S proteasome at the center of the ubiquitin-proteasome system (UPS) is essential for virtually all cellular processes of eukaryotes. A common misconception about the proteasome is that, once made, it remains as a static and uniform complex with spontaneous and constitutive activity for protei...
The COP9 signalosome (CSN) is intimately connected to the ubiquitin/26S proteasome system and might regulate the activity of SCF-type (and possibly other) E3 ligases. Recent advances, particularly in the fields of hormone signalling and responses to pathogens, have shown just how frequently plants...
Proteasomal regulation Transcriptional regulation of proteasome biogenesis Current knowledge regarding the basal rate of prote- asome subunit biosynthesis is limited. It is believed that all subunits of the "canonical" proteasome are found in cells only in the context of their respective complex, the ...
The proteasome inhibitor MG132 (2.5 μM) was used to inhibit the degradation of ubiquitinated proteins. W, wild type; K, (A) Wdr26-knockout or (B) Rmnd5a-knockout. (C) Ubiquitination of Lamin B was alleviated in Wdr26-knockout MEL cells. *Lamin B. (D) Decreased rate of Lamin B...
The 26S proteasome is a large, ∼2.5 MDa, multi-catalytic ATP-dependent protease complex that serves as the degrading arm of the ubiquitin system, which is the major pathway for regulated degradation of cytosolic, nuclear and membrane proteins in all e
Here, we describe a new strategy of targeted protein degradation through direct substrate recruitment to the 26S proteasome. The proteolytic complex is essential and abundantly expressed in all cells; however, proteasomal ligands remain scarce. We identify potent peptidic macrocycles that bind directly ...
The 26S proteasome is the most complex ATP-dependent protease machinery, of ~2.5 MDa mass, ubiquitously found in all eukaryotes. It selectively degrades ubiquitin-conjugated proteins and plays fundamentally indispensable roles in regulating almost all