2-NBDG can be used to monitor glucose uptake in living cells and tissues by real-time confocal, high-resolution, or wide-field fluorescence microscopy and flow cytometry. 体外活性 方法:Flow cytometry 检测葡萄糖摄取:1、细胞按 1*104/孔种于96孔板,24-48 h 内进行实验。2、去除细胞培养基,加入含...
2-NBDG (MKBio, Shanghai, China, CAS NO: 186689-07-6), a green,fluorescent deoxyglucose analog, was used to monitor the cell glucose uptake using flow cytometry or fluorescence microscopy, as an indicator of cellular viability. To detect the uptake of 2-NBDG, 1 × 105cells/well were see...
2-NBDG can be used in real-time confocal, high-resolution, or wide-field fluorescence microscopy as well as in flow cytometry。 The probe can be excited by the Argon laser at 488 nm to give the environment-sensitive fluorescence。 It has lower photostability than the rhodamine-based ...
2-NBDG can be used in real-time confocal, high-resolution, or wide-field fluorescence microscopy as well as in flow cytometry. 应用: 产品信息订购: 产品货号 产品名称 规格 价格 大包装及货期 abs47045483 2-NBDG 1mg 970.00 立即咨询 产品更多信息请进入爱必信网站咨询 公司简介 爱必信(上海)生物科技...
Flow cytometry was performed to visualize uptake in intact cells, and a fluorescence microplate reader was used to measure the intracellular concentration of 2-NBDG in cell homogenates. 2-NBDG uptake was concentration dependent in astrocytes that were exposed or not exposed to OGD. OGD significantly...
Analyze cells using fluorescence microscope or flow cytometer with 530/30 nm filter (FITC channel) Important notes Thaw all the components at room temperature before starting the experiment. PREPARATION OF WORKING SOLUTION Add 5 µL of 2-NBDG (10 mg/mL) (Component A) to 1.5 mL of Assay Bu...
by flow cytometry. G F H 2.6 运用2-NBDG检测胰岛素处理H9c2心肌细胞后细胞葡萄糖摄取的能力 运用胰岛素(100 uM)处理 H9c2 心肌细胞。H9c2 心肌细胞自发荧光强度为 3.82±0.23;2-NBDG 组荧光强度为 78.13±10.96,显著高于对照组(P<0.01); 2-NBDG+胰岛素组荧光强度为109.09±11.67,较单纯2-NBDG 组增加39.6...
Fluorescence analysis of 2-NBDG uptake by flow cytometry.R. DhanyaK. B. ArunV. M. NishaH. P. SyamaP. NishaT. R. Santhosh KumarP. Jayamurthy
itiveinhibition.Flowcytometryanalysisof2一NBDGuptakedisplayedrapiduptakeforthefirstoneminute (theFluorescencebackgroundofMDA—MB-231cellswas 2 .66 ±0 .09 .incubation with 2 -NBDG fo r 1 mi— nute was 4.04 ±0.18 ) ,the absorption of 2一NBDG incubation for 10 minutes continued to increasely( fl...
2-NBDG is a fluorescent glucose analog for visualizing glucose uptake into living cells. Optical Data for 2-NBDG Emission Color Green λabs 467 nm λem 542 nm Application Flow Cytometry, Confocal Microscopy Plan Your Experiments Use our spectra viewer to interactively plan your experiments, assess...