2-NBDG can be used in real-time confocal, high-resolution, or wide-field fluorescence microscopy as well as in flow cytometry。 The probe can be excited by the Argon laser at 488 nm to give the environment-sensitive fluorescence。 It has lower photostability than the rhodamine-based ...
that is used primarily to directly monitor glucose uptake by living cells and tissues. It is also used as a topical contrast reagent for the detection of neoplasia. 2-NBDG can be used in real-time confocal, high-resolution, or wide-field fluorescence microscopy as well as in flow cytometry....
2-NBDG can be used to monitor glucose uptake in living cells and tissues by real-time confocal, high-resolution, or wide-field fluorescence microscopy and flow cytometry. 体外活性 方法:Flow cytometry 检测葡萄糖摄取:1、细胞按 1*104/孔种于96孔板,24-48 h 内进行实验。2、去除细胞培养基,加入含...
2-NBDG (MKBio, Shanghai, China, CAS NO: 186689-07-6), a green,fluorescent deoxyglucose analog, was used to monitor the cell glucose uptake using flow cytometry or fluorescence microscopy, as an indicator of cellular viability. To detect the uptake of 2-NBDG, 1 × 105cells/well were see...
Fluorescence analysis of 2-NBDG uptake by flow cytometry.R. DhanyaK. B. ArunV. M. NishaH. P. SyamaP. NishaT. R. Santhosh KumarP. Jayamurthy
Flow cytometry was performed to visualize uptake in intact cells, and a fluorescence microplate reader was used to measure the intracellular concentration of 2-NBDG in cell homogenates. 2-NBDG uptake was concentration dependent in astrocytes that were exposed or not exposed to OGD. OGD significantly...
itiveinhibition.Flowcytometryanalysisof2一NBDGuptakedisplayedrapiduptakeforthefirstoneminute (theFluorescencebackgroundofMDA—MB-231cellswas 2 .66 ±0 .09 .incubation with 2 -NBDG fo r 1 mi— nute was 4.04 ±0.18 ) ,the absorption of 2一NBDG incubation for 10 minutes continued to increasely( fl...
Analyze cells using fluorescence microscope or flow cytometer with 530/30 nm filter (FITC channel) Important notes Thaw all the components at room temperature before starting the experiment. PREPARATION OF WORKING SOLUTION Add 5 µL of 2-NBDG (10 mg/mL) (Component A) to 1.5 mL of Assay Bu...
第34卷第15期 第三军医大学学报 Vol.34,No.15 2012年8月15日 J Third Mil Med Univ Aug.15 2012 1533 文章编号:1000 5404(2012)15 1533 05 论著 2 NBDG检测H9c2心肌细胞葡萄糖摄取能力的研究 叶林,陆凯,张冬颖,覃数 (400016重庆,重庆医科大学附属第一医院心血管内科) [摘要] 目的 评估荧光标记2 脱氧葡...
'BACKGROUND: We investigated the effects of insulin on glucose transport in human monocytes using flow cytometry, a method with several advantages over previously used techniques. We hypothesized that monocytes could be used as tools to study insulin action at the cellular level and facilitate the ...