Using cryo-electron microscopy, we find thatSgEnc forms an icosahedral protein shell and encapsulates 2-methylisoborneol synthase (2-MIBS) as a cargo protein.SgEnc contains a cyclic adenosine monophosphate (cAMP) binding domain (CBD)-fold insertion and a unique metal-binding domain, both ...
More specifically, transcription of one geosmin synthase gene (geoA) and two 2-MIB synthase genes (mtfandmtc) was explored. Of the three temperatures (15, 25, and 35 °C) tested, the maximum Chl-acontent was determined at 25 °C in bothD. smithiiandP. foetidavar.intermedia. The ...
Based on the identity, geosmin sequences were found to be more diverse in the geosmin synthase conserved/primer design region, compared to 2-MIB synthesis region, hindering the design of universal primers/probes. Emerging technologies such as the bioelectronic nose, Surface Enhanced Raman Scattering...
MIB synthaseOff-flavorsRainbow troutRecirculating aquaculture sytem (RAS)qPCRIn recirculating aquaculture systems (RAS)s, off-flavors and odors, mainly caused by geosmin (GSM) and 2-methylisoborneol (MIB), can accumulate in the flesh of fish from RAS water, reducing the profitability of production...