5-Ketogluconate reductase (5KGR) from the cell free extract of Gluconobacter liquefaciens (IFO 12388) was partially purified about 120-fold by a procedure employing ammonium sulfate fractionation, and DEAE-cellulose-, hydroxylapatite- and DEAE-Sephadex A-50- column chromatographies. NADP was ...
The crystalline enzyme and crude enzyme preparations of 2-keto-o-gluconate reductase is stable in 0.01 M potassium phosphate, pH 6.0, containing 1 m M 2-mercaptoethanol . Enzyme activity for the reduction of 2-keto-D-gluconate to D-gluconate is found at pH 6.0 with all enzyme preparations ...
In this study, we investigated the anoxic electrogenic phenotype ofP. putidaKT2440 at a systems level. We applied multi-omics profiling during the bio-electrochemical oxidation of glucose, integrating the analysis of metabolites, proteins, and transcripts to uncover previously unexplored metabolic reaction...
Cytochrome c reductase is a key enzyme involved in the extracellular electron transfer pathway towards transition metal complexes in Pseudomonas putida. Chemsuschem. 2020;13:5308–17. Article CAS PubMed PubMed Central Google Scholar Culviner Peter H, Guegler Chantal K, Laub Michael T. A simple,...
2-Ketogluconate reductase (EC 1. 1. 99. 3) has been crystallized for the first time from the cell free extract of Gluconobacter liquefaciens IFO 12388. We believe that this enzyme prep aration is the first crystalline enzyme which has been crystallized from the acetic acid bacteria. G. ...
CONSTITUTION: The yafB gene codes the 2,5-diketo-gluconate reductase gene for production of 2-keto-D-gluconate, which is originated from E. coli. The yafB gene is inserted into a recombinant plasmid pYAFB. The recombinant plasmid pYAFB with the yafB gene inserted thereinto is transformed ...
2-Ketogalactonate reductaseNADPNADPHPseudomonas fluorescens2-Keto- d-gluconate and 2-keto- d-galactonate were prepared from d-glucose (with a yield of 40%) and d-galactose (with a yield of 25%), respectively, with cell culture of Pseudomonas fluorescens. However, 2-keto- d-mannoate was ...
reductaseNADPNADPHPseudomonasfluorescens2-Keto-d-gluconate and 2-keto-d-galactonate were prepared from d-glucose (with a yield of 40%) and d-galactose (with a yield of 25%), respectively, with cell culture of Pseudomonas fluorescens. However, 2-keto-d-mannoate was not prepared in this ...
Short-chain dehydrogenase/reductase (SDR) is distributed in many organisms, from bacteria to humans, and has significant roles in metabolism of carbohydrates, lipids, amino acids, and other biomolecules. An important intermediate in acidic polysaccharide metabolism is 2-keto-3-deoxy-D-gluconate (KDG...
Ameyama M, Chiyonobu T, Adachi O (1974) Purification and properties of 5-ketogluconate reductase from Gluco- nobacter liquefaciens. Agric Biol Chem 38:1377-1382.Ameyama M, Chiyonobu T, Adachi O. Purification and properties of 5-ketogluconate reductase from Gluconobacter liquefaciens[J]. ...