This chapter discusses the determination of 2-Keto-3-deoxy-6-phosphogluconate (KDPG). The two enzymatic methods are described. In the first method 6-phosphogluconate is dehydrated with the enzyme 6-phosphogluconic dehydrase. This has the disadvantage that dehydrase must be free of KDPG aldolase. ...
The pyruvate aldolases use pyruvate as the nucleophilic component in stereoselective aldol condensations, producing a 4-hydroxy-2-ketobutyrate framework. We have examined the 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolases from Pseudomonas putida, Escherichia coli, and Zymomonas mobilis for utility...
2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolasedoi:10.1002/9783527809080.cataz09401C.-H. WongAmerican Cancer Society
The molecular weights of native 2-keto-3-deoxy-6-phosphogluconate aldolase and its subunit have been determined. The following physical constants have been calculated for the native protein: S 0 20 w = 4.35 S ± 0.06 S ; D 0 20, w = 5.60 ± 0.10 x 10 -7 cm 2 sec -1 ; molecular...
Kim J, Yeom J, Jeon CO & Park W (2009) Intracellular 2-keto-3- deoxy-6-phosphogluconate is the signal for carbon catabolite repression of phenylacetic acid metabolism in Pseudomonas putida KT2440. Microbiology 155: 2420-2428.Juhyun Kim Jinki Yeom Che Ok Jeon . Intracellular 2-keto-3-deoxy...
2001 . Covalent intermediate trapped in 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase structure at 1.95 Å resolution . Proc. Natl. Acad. Sci. 98 : 3679 – 3684 .J. Allard, P. Grochulski, J. Sygusch, Covalent intermediate trapped in 2-keto-3-deoxy-6- phosphogluconate (KDPG) ...
2001 . Directed evolution of a new catalytic site in 2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli . Structure 9 : 1 – 9 .Wymer N, Buchanan LV, Henderson D, Mehta N, Botting CH, Pocivavsek L, Fierke CA, Toone EJ, Naismith JH: Directed evolution of a new ...
D-Glyceraldehyde is accepted as an electrophile by 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase (EC 4.1.2.14) at 1% the rate of natural substrate, D-glyceraldehyde 3-phosphate. Accordingly, it was expected that addition of a phosphate moiety at C3 or C4 of unnatural aldehydes would ...
The genes encode a 2-keto-3-deoxy-6-phosphogluconate (KDPGA) and a fructose bisphosphate aldolase (FFBPA), as well as numerous other genes. The genes will be useful in C1 metabolizing microorganisms for the manipulation of the carbon flux pathway.Mattheos Koffas...
2-Keto-3-deoxy-6-phosphogluconate (KDPG) aldolase from Pseudomonas putida is examined for its utility as a biocatalyst in the stereoselective aldol condensation between pyruvate and aldehydes of varying steric and electronic structure. KDPG aldolase has been used to prepare stereospecific aldol ...