2-Hydroxyisoflavanone synthase substantially having the amino acid sequence represented by SEQ ID NO:2 and a polynucleotide substantially having the nucleotide sequence of SEQ ID NO:1. Thus, 2-hydroxyisoflavanone synthase can be expressed in a host cell. By transferring the polynucleotide encoding ...
(2002) Key amino acid residues required for aryl migration catalysed by the cytochrome P450 2-hydroxyisoflavanone synthase. Plant J. 31: pp. 555-564Sawada, Y.; Kinoshita, K.; Akashi, T.; Aoki, T.; Ayabe, S.: Key amino acid residues required for aryl migration catalysed by the ...
licorice 2-hydroxyisoflavanone synthase (IFS) and soybean 2-hydroxyisoflavanone dehydratase (HID) catalyzing sequential reactions to yield isoflavones. While the control and the IFS overexpressor did not accumulate detectable isoflavones, the HID overexpressors did accumulate daidzein and genistein, ...
2-Hydroxyisoflavanone synthasedoi:10.1007/978-3-540-85188-2_83Schomburg, DietmarSchomburg, IdaChang, AntjeSpringer Berlin Heidelberg
2-Hydroxyisoflavanone, the substrate of the dehydratase, is the product of 2-hydroxyisoflavanone synthase, as cytochrome P-450 which catalyzes the hydroxylation step associated with aryl migration of flavanone. The dehydratase was purified to apparent homogeneity for the first time by a seven-step ...
2-Hydroxyisoflavanone synthase substantially having the amino acid sequence represented by SEQ ID NO:2 and a polynucleotide substantially having the nucleotide sequence of SEQ ID NO:1. Thus, 2-hydroxyisoflavanone synthase can be expressed in a host cell. By transferring the polynucleotide encoding ...
Akashi T, Aoki T, Ayabe S (1999) Cloning and functional expression of a cytochrome P450 cDNA encoding 2-hydroxyisoflavanone synthase involved in biosynthesis of the isoflavonoid skeleton in licorice. Plant Physiol 121:821–828Akashi T, Aoki T, Ayabe S-i (1999) Cloning a...
Here, we analyzed binary protein鈥損rotein interactions of 2-hydroxyisoflavanone synthase 1 (GmIFS1), a P450 (CYP93C), with cytoplasmic enzymes involved in isoflavone biosynthesis in soybean. We identified binary interactions between GmIFS1 and chalcone synthase 1 (GmCHS1) and between GmIFS1 ...