Universal Direct Taqman qPCR Master Mix(2×) 产品描述 产品简介 本制品为采用探针法(Probe)进行Real Time PCR(qPCR)反应的专用试剂。产品经过了特殊制备工艺,完全去除了宿主DNA残留;经过优化的配方,可被制成冷冻干燥制剂;适用于针对血液、唾液、拭子、组织、植物、微生物等样本提取的基因组DNA、病毒DNA、质粒DNA...
For all PCR reactions, 1 μl of cDNA was added to 10 μl TaqMan 2× Universal PCR Mastermix Mix (Applied Biosystems), 1 μl of each primer [20 μM], 1 μl 5'-FAM-labeled probe [0.3 μM] and 8 μl deionized H2O. All reactions were performed in duplicates and all three ...
Quantification of transcription levels relative to β-Actin (inner control) was performed using TaqMan Universal Master Mix II, with UNG (Applied Biosystems), according to the manufacturer’s protocol and utilizing the following gene expression assays: Mm02619580_g1 Actb 4448484 TaqMan Gene Expression...
TaqMan probes for Alp (Mm01187117_m1), Ibsp (Mm00492555_m1), Bglap (Mm03413826_mH), Pdgfra (Mm00440701) and Gapdh (Mm99999915_g1) with TaqMan Universal Mastermix were used to evaluate gene expression. Pdgfrb, Noggin, Id1 and Dxl5 expression were evaluated using a SYBR green-based assay...
2.英文名:Universal primers ITS2 (10uM) 3.货号:PC2472-250 4.品牌:solarbio 5.储存条件:-20℃ 产品用途:PCR 引物。 相关产品: PC1100-5000Taq DNA PolymeraseSolarbio5000U PC1120-52×Taq PCR MasterMix (含染料)Solarbio5×1ml PC1150-52×Taq PCR MasterMix (不含染料)Solarbio5×1ml ...
Real-time PCR was performed using TaqMan Fast Universal PCR Master Mix, No AmpErase UNG in the Step One Plus Real-Time PCR System (Life Technologies, Carlsbad, CA). Flow cytometry and cell sorting Single-cell suspensions were prepared from thymus and bone marrow (femoral and tibial bones) ...
Reactions (20 μl) containing 9 μl of cDNA, primer/probe pairs for TFPI2 (Hs00197918_m1), obtained from Assay-on-demandTM (Applied Biosystems, Foster City, USA) and TaqMan Universal PCR Master Mix solution (Applied Biosystems, Foster City, USA), were run in duplicate. A threshold cycle...
TaqMan real-time polymerase assays were performed utilizing TaqMan Universal PCR Master Mix (4304437, Applied Biosystem) and the following TaqMan Gene Expression assays: Bcl-2 (Mm00477631_m1, Applied Biosystems); Caspase-3 (Mm01195085_m1, Applied Biosystems); Leptin Receptor (Mm00440181_m1, Applied...
qPCR was performed using a StepOnePlusTM system, predesigned TaqMan® Gene Expression Assays and TaqMan® Universal Master Mix II (all from ThermoFisher Scientific). Singleplex reactions contained 1 μL of the predesigned gene expression assay (Rem2, Mm00600529_m1; Actb, 4352933E; Gapdh, ...
Total RNA (100 ng) was used to generate cDNA using TaqMan Universal Master Mix. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed using an ABI Prism 7300 Sequence Detection System (Applied Biosystems, Foster City, CA, USA). Beta-actin (Mouse ACTB, 4352341E) was used ...