2×Taq plus Master Mix专为常规PCR扩增反应优化,使用时只需再加入模板和引物并用水补足体系至反应浓度为1×,即可进行PCR反应,大大地简化了操作过程,减少了PCR操作过程中的污染。经测试,染料的加入不影响PCR反应,在PCR反应完成后可直接电泳,节省时间。使用本试剂扩增得到的PCR产物3´端有一突出“A”碱基,可直接...
PRKAA2 mRNA levels were measured using quantitative real-time PCR (qPCR). Total RNA was extracted using an RNA extraction reagent (Takara, 9108, Japan), and 2X Super SYBR Green qPCR Master Mix (ES Science, Guangzhou, China) was used to detect PRKAA2 levels. The qPCR primer sequences ...
The following RT–PCR kits were tested to determine the best performing assay: (1) OneStep RT–PCR Kit (QIAGEN); (2) TaqPath 1-Step Multiplex Master Mix (Thermo Fisher Scientific); (3) One-Step PrimeScript RT–PCR Kit (Takara Bio); (4) GoTaq Probe qPCR Kit (Promega Corporation); (...
PCR products were subcloned into the pTA2 vector (TOYOBO) and sequenced. Real-time RT–PCR analysis. Total RNA was isolated from cells using TRIzol reagent (Invitrogen) and 1 mg of total RNA was reverse transcribed to obtain first- strand cDNA using an RNA-PCR kit (Takara) following the ...
2.7. Quantitative real-time PCR (qRT-PCR) analysis RNA in the cell culture supernatant was extracted with Libex 96 (qEx-DNA/RNA, T183X) and used for reverse transcription by PrimerScript RT Master Mix (Takara, Lot:AN82328A). qRT-PCR was performed on a CFX Connect (Bio-rad) using iTa...
Viral RNA was translated to cDNA by PrimeScript™ RT Master Mix (TaKaRa, Dalian, China) before PCR. Each sample test was repeated three times. Sensitivity of the PCR-LFIA The genomic DNA of CPV-2-N1 strain was extracted for PCR amplification. PCR products were purified by Magbeads PCR ...
1-哌啶实现了低背景的高灵敏度PCR反应 L-4,4'-联苯氨TaKaRa Taq™ HS Low DNA 4-AMINO-N-(TERT-BUTYL)BENZAMIDE实现了低背景的PCR反应 2-(4-Nitrophenyl)-2-propanolTks Gflex™ DNA Polymerase Low DNA 1-异氰基苯室温保存的PCR Master Mix ...
Freezing and thawing were repeated two more times to lyse the cells, and 2 units of recombinant DNase I (Takara Bio) was added to the solution. The solution was incubated at room temperature until the solution became fluid due to DNA degradation. The solution was then centrifuged at 12 000...
Macropinocytosis is essential for myeloid cells to survey their environment and for growth of RAS-transformed cancer cells. Several growth factors and inflammatory stimuli are known to induce macropinocytosis, but its endogenous inhibitors have remained
As the COVID-19 infection continues to ravage the world, the advent of an efficient as well as the economization of the existing RT-PCR based detection assay essentially can become a blessing in these testing times and significantly help in the management of the pandemic. This study demonstrat...