The analytical sensitivity of the real-time RPA was 101 copies/reaction of a standard DNA template, which was 10 times more sensitive than the common RPA method. The clinical sensitivity of the real-time RPA assay matched 100% (n = 91) to the real-time PCR results. Conclusion The real-...
Quantitative reverse transcription PCR (qRT-PCR) Total RNA was extracted by TRIzol (Invitrogen) and 5 μg RNA was used to generate complementary DNA. Transcriptional levels of genes were determined by Premix Ex Taq (Takara) or ChamQ SYBR qPCR Master Mix (Vazyme Biotech Co., Ltd) and analyz...
TSG (100, 200, 400, 800 mg/kg) enhanced liver CYP1A2 catalytic activity when mice were treated with different doses of TSG for 12 h (Fig.4E). When mice were treated with TSG (800 mg/kg) for different times, TSG increased liver CYP1A2 catalytic activity after treated mice for...
Adenosine monophosphate-activated protein kinase (AMPK) is associated with the development of liver hepatocellular carcinoma (LIHC). AMPKα2, an α2 subunit of AMPK, is encoded by PRKAA2, and functions as the catalytic core of AMPK. However, the role of
RNA preparation and quantitative real-time PCR Total RNA was extracted according to the manufacturer’s instructions. RT-qPCR was performed using a SYBR Premix Ex Taq (TaKaRa) on a Thermal Cycler Dice Real Time System (TaKaRa) with the following protocol: 30 s at 95°C followed by two-step...
10 μl of GoTaq qPCR Master Mix, and 0.5 μl of each primer was used and adjusted to the final volume with RNase-free water. β-actin in parallel for each run was used as an internal control. The reactions were set up on the basis of the manufacturer’s protocol. PCR conditions wer...
Viral RNA was translated to cDNA by PrimeScript™ RT Master Mix (TaKaRa, Dalian, China) before PCR. Each sample test was repeated three times. Sensitivity of the PCR-LFIA The genomic DNA of CPV-2-N1 strain was extracted for PCR amplification. PCR products were purified by Magbeads PCR ...
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cDNA primer with OligodT in a 20 ml reaction mix using 200 U/ml of PrimeScript M-MuLV reverse transcriptase (Takara Bio Inc., USA) following the manufacturer’s instructions. Quantitative real time (qRT) PCR was performed using SYBR Premix ExTaq II (TliRNaseH Plus) (Takara Bio Inc., ...
As the COVID-19 infection continues to ravage the world, the advent of an efficient as well as the economization of the existing RT-PCR based detection assay essentially can become a blessing in these testing times and significantly help in the management of the pandemic. This study demonstrat...