2-NBDG 是一种荧光葡萄糖类似物,已经用于监测活细胞中的葡萄糖摄取情况,可作为细胞活力的指示剂。尽管对环境敏感,但 NBD 荧光通常在 ∼465/540 波长处出现最大激发/发射,并且可以使用专为荧光素设计的滤光片显示。了解更多信息 Have Questions? 货号数量 ...
一、HNF1α促进肾相关细胞葡萄糖类似物的重吸收作用在HK2和HEK293T细胞中分别转染空载体pcDNA3.1myc-HisB为对照组,pcDNA3.1-HNF1α-myc为实验组,分别用含Na+和不含Na+的缓冲液与荧光葡萄糖类似物2-NBDG共同避光孵育后用流式细胞...
During this process, 2-NBDG, a glucose analog, was used to evaluate the cells' glucose uptake capacity. After incubation, specific antibodies targeting surface markers of HSCs were added, and cellular uptake of 2-NBDG was quantitatively analyzed using FACS. Intracellular ATP levels are measured ...
(Gibco, 10099-141), 2-脱氧葡萄糖(Sigma-Aldrich, D8375), 2-(N-7-硝基-2,1,3-苯并二唑-4-氨基)-2-脱氧葡萄糖 (2-NBDG, Thermo Fisher Scientific, N13195), Annexin/PI染色试剂盒(Biotool, B32115), 流式细胞分析仪(CytoFLEX S), 激光共聚焦显微镜 (Nikon A1 Spectral Confocal Microscope), ...
T cells from healthy donor peripheral blood mononuclear cells were purified by negative selection using a T cell isolation kit (Miltenyi) and subsequently activated with CD3/CD28 Dynabeads (Thermo Fisher Scientific) at a cell-to-bead ratio of 1:3. A lentiviral vector carrying a previously ...
2-NBDG Thermo Fisher Scientific Cat# N13195 Critical Commercial Assays ATP Somatic Cell Assay Kit Sigma-Aldrich Cat# FLASC-1KT Lactate Colorimetric/Fluorometric Assay Kit BioVision Cat# K607 RT2 Profiler PCR Array Human AMPK Signaling QIAGEN Cat# PAHS-175Z Milliplex human Th17 25-plex kit Milli...
2-NBDG 价格￥1300 起批量≥1毫克 最小起订0.5毫克 供货总量0.5毫克 发货地址 建议售价￥1300/毫克 更新日期2025年05月09日 即时洽谈立即询价查看联系方式 收藏产品发送留言 VIP11年 上海惠诚生物科技有限公司 企业认证:备 营业执照和法人身份证已备案
After 5 h incubation at 37 °C/5% CO2, 5, 5′, 6, 6′-tetrachloro- 1, 1′, tetraethylbenzimidazolocarbocyanine iodide (JC-1, mitochondrial membrane potential detection indicator, ThermoFisher Scientific) and NucBlue® Live ReadyP- robes (Nuclear staining solution, ThermoFisher Scien- tific...
2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose) was purchased from ThermoFisher (Carlsbad, CA). 2.1.5. Pure peptides Pure peptides, purity greater than 98%, were synthetized by Genescript (Piscataway, NJ). Peptides AKSPLF, ATNPLF, FEELN, LSVSVL as origina...
Mouse BV2 microglia cells, Dulbecco's modified Eagle's medium (DMEM, Gibco, 11965-118), fetal bovine serum (Gibco, 10099-141), 2-deoxy-D-glucose (Sigma-Aldrich, D8375), 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxy-D-glucose (2-NBDG, Thermo Fisher Scientific, N13195...