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英文名称:2-NBDG Glucose Uptake Assay Kit (Cell-Based) 齐一生物专业代理BioVision产品,齐一生物更快捷的到货时间,我们将以更短的订货周期,为您提供*质产品。齐一生物销售全国免费齐一生物欢迎您咨询更多的产品 齐一生物专业代理美国BioVision产品,BioVision 公司是世界*的细胞凋亡和信号传导产品生产商,BioVision在...
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Upon completion of pre-treatment, 50 nM of 2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino]-2-Deoxyglucose (2-NBDG) (provided by Invitrogen, Cat. No. N13195) was added to each well and incubated at 37 °C for 30 min. During this process, 2-NBDG, a glucose analog, was used ...
2-NBDG injection The mice were maintained for 72 h post-TBI, with a 16-hour fasting period preceding tail vein injection of 2-NBDG at a dose of 375 µg per mouse (storage solution concentration: 2.5 mg/mL; injection volume: 150 µL per mouse) [33]. One-hour postinjection, the ...
荧光成像显示2-NBDG积聚在MDA—MB231细胞膜和细胞质内,加入D型葡萄糖竞争抑制后,荧光信号显著减弱;流 式细胞仅分析表明最初的1min2-NBDG被肿瘤细胞迅速摄取(MDA—MB一231细胞自发荧光强度为2.66±0.09,2-NB— DG孵育1min后荧光强度为4.04±0.18),10min时吸收持续增加(荧光强度为5.64±0.17),20min吸收最明显(荧...
仪等多种实验方法观察分析H9c2心肌细胞摄取2-NBDG的能力,探索2-NBDG作 为荧光探针检测心肌细胞葡萄糖摄取的可行性。 1 材料与方法 1.1 主要材料及仪器 DMEM/F12 细胞培养基、胎牛血清(Hyclone,America);2-NBDG(Invitrogen, America);Anti- GLUT1、Anti-GLUT-4 (北京博奥森生物技术有限公司);FITC 标 记羊抗...
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产品线Invitrogen 激发/发射469/537 nm Unit SizeEach 图表 Chemical Structure 荧光光谱 在SpectraViewer 中打开 文件和下载 证书 搜索 批号Certificate TypeDateCatalog Number(s) 3165168Certificate of Analysis2025年3月31日N1148 2978498Certificate of Analysis2024年8月28日N1148 ...
The annexin V–FITC (A23204, Invitrogen) and propidium iodide (PI) (P4864, Sigma-Aldrich) double staining technique was used to evaluate apoptosis. Cells were seeded in 6-well plates at a density of of 1 × 105cell/mL. A positive control was performed by treating cells with 1 ...