2-aminomuconic 6-semialdehyde dehydrogenase (AmnC), a member of the aldehyde dehydrogenase (ALDH) superfamily, is responsible for oxidizing 2-aminomuconic 6-semialdehyde to 2-aminomuconate. In contrast to many other members of the ALDH superfamily, the structural basis of the catalytic activity of...
A method for the preparation of 2-aminomuconate which comprises adding 2-aminophenol to a mixture of 2-aminophenol 1,6-dioxygenase, 2-aminomuconic semialdehyde dehydrogenase and NAD (nicotinamide adenine dinucleotide, oxidized form) in a buffer, and recovering a fraction containing 2-aminomuconate fr...
2-Hydroxymuconic semialdehyde dehydrogenase was included since it is a dimer like the scallop ALDH/crystallin, unlike the other tetrameric native ALDH1/2 proteins. Download: Download high-res image (243KB) Download: Download full-size image Figure 5. Amino acid sequence alignment of the deduced ...
pseudoalcaligenes JS45. The 2-aminophenol 1, 6-dioxygenase genes are located upstream of the 2-aminomuconic semialdehyde dehydrogenase gene, and a putative member of the YjgF protein family is u 展开 关键词: Key wordsPseudomonas 2-Aminophenol Catechol Dioxygenase meta-Cleavage pathway Biodegradation ...
The xylC product, the genuine benzaldehyde dehydrogenase, was purified from extracts of P. putida (pWW0-161 delta rylG) which does not synthesize 2-hydroxymuconic semialdehyde dehydrogenase. The amino-terminal sequence of the purified protein corresponds to the amino-terminal sequence deduced from ...
DJ77phnGGene Encoding 2-Hydroxymuconic Semialdehyde Dehydrogenase The nucleotide sequence of a 1520 bp region, spanning the coding region for themeta-cleavage pathway enzyme, 2-hydroxymuconic semialdehyde dehydrogenase, w... Sungje,Kim,and,... - 《Biochemical & Biophysical Research Communications》 ...
C23O activity was measured with standard method by determining the formation of 2-hydroxymuconic semialdehyde spectrophotometrically at 375 nm (λ375 = 36,000 M−1cm−1), in a reaction mixture containing 20 μL of catechol (50 mM), 960 μL of phosphoric buffer pH 7.4 (...
The deduced amino acid sequences of the PhnI (312 amino acids) and PhnJ (343 amino acids) have identities of 51-71% with the corresponding genes of dmp, xyl, nah, bph_LB400, bph_KKS102, tod, cum, cmt, and MTCY03C7 operons. The phylogenetic analyses reveal the evolutionary ...
This observation rules out the formation of 2-hydroxy-4-carboxymuconic semialdehyde, the meta-cleavage product [40] of PCA, which is yellow in color. Thus, it is assumed that PCA was degraded by ortho-cleavage dioxygenase, and this was confirmed by the spectrophotometric determination of the ...
Isolation and Identification of the Reaction Product of α-Hydroxy-γ-Carboxymuconic ε-Semialdehyde Dehydrogenase The reaction product of enzymic dehydrogenation of ar-hydroxy-r-carboxymuconic e-semial-dehyde (HCMS) was isolated. The analytical data (elemental analyses... Kiyofumi MARUYAMA - 《J Bi...