2013b. Synthetic 2,3-butanediol pathway integrated using Tn7-tool and powered via elimination of sporulation and acetate production in acetogen biocatalyst. Appl Biochem Biotechnol 170(6):1503-24.Tyurin M, Kiriukhin M. Synthetic 2,3-butanediol pathway integrated using Tn7-tool and powered via ...
近日来自慕尼黑工业大学的JochenSchmid等人在Metabolic Engineering上发表的文章《Engineering of the 2,3-butanediol pathway ofPaenibacillus polymyxaDSM 365》通过代谢工程的方法提高了多粘芽胞杆菌DSM 365中的2,3-丁二醇产量。 文章中,作者通过CRISPR-Cas9介导的基因组编辑,获得了6个代谢突变体,并在批量发酵中对这6个...
Butanediol is produced in a mixed acid fermentation pathway starting from pyruvate. Other side products such as lactate, ethanol, acetate or formate are competing over pyruvate as a substrate. Blue: polysaccharide synthesis, green: butanediol pathway, purple: mixed acid pathway, sacB: levansucrase, ...
Some fermentative organism catabolize glucose by the butanediol pathway, in which acetoin (acetylmethylcarbinol) occurs as an intermediate in the formation of 2,3-butanediol. In the presence of KOH and O2, the acetoin is oxidized to diacetyl, which in turn reacts with the guanidine group associa...
Overflow metabolism-caused acetate accumulation is a major problem that restricts industrial applications of various bacteria. 2,3-Butanediol (2,3-BD) synthesis in microorganisms is an ancient metabolic process with unidentified functions. We demonstrate
pneumoniae , the 2,3-BDO synthesis pathway is dominated by three essential enzymes, namely acetolactate decarboxylase, acetolactate synthase, and butanediol dehydrogenase, which are encoded by the budA , budB, and budC genes, respectively. The mechanisms of the three enzymes have been characterized ...
Finally, 2,3-butanediol fermentation research directions are proposed.%能源危机和环境污染使得化 工行业的发展举步维艰,亟待开发新的发展模式,以可再生能源为原料的生物炼制技 术成为可行的途径之一.2,3-丁二醇的发酵生产是现代生物炼制的重要课题之 一.2,3-丁二醇作为一种大宗的化学产品具有广泛的应用价值,...
Based on the best in silico strategy, in which disruption of alcohol dehydrogenase (ADH) pathway is required, we then constructed gene deletion mutant strains and performed batch cultivation of the strains. Deletion of three ADH genes, ADH1, ADH3 and ADH5, increased 2,3-butanediol production ...
Identification of acetoin reductases involved in 2,3-butanediol pathway in Klebsiella oxytoca. J. Biotechnol. 172, 59–66 (2014). 19. Rao, B. et al. Characterization and regulation of the 2,3-butanediol pathway in Serratia marcescens. Appl. Microbiol. Biotechnol. 93, 2147–2159 (2012). ...
to the genes in the bud manipulation,ilvB and ilvI also encode the acetylactate synthase in the synthesis pathway of valine,and three dehydrogenases of dhaD,gldA and acyI are reported to have the activity of butanediol dehydrogenase.However,the role of these en zymes on 2,3-butanediol ...