近日来自慕尼黑工业大学的JochenSchmid等人在Metabolic Engineering上发表的文章《Engineering of the 2,3-butanediol pathway ofPaenibacillus polymyxaDSM 365》通过代谢工程的方法提高了多粘芽胞杆菌DSM 365中的2,3-丁二醇产量。 文章中,作者通过CRISPR-Cas9介导的基因组编辑,获得了6个代谢突变体,并在批量发酵中对这6个...
In this study, endogenous capability of Escherichia coli for 2,3-butanediol (2,3-BD) synthesis was explored using the anaerobic selection platform based on redox balance. To address the issue of NADH excess associated with the 2,3-BD pathway, we devised a substrate-decoupled system where a ...
Paenibacillus polymyxa is a Gram-positive, non-pathogenic soil bacterium that has been extensively investigated for the production of R-,R-2,3-butanediol in exceptionally high enantiomeric purity. Rational metabolic engineering efforts to increase productivity and product titers were restricted due to lim...
按照表3中反应体系,加入1 mg/L的纯酶,测得2,3-丁二醇脱氢酶plaC相对于meso-2,3-butanediol的Km为3.42 mmol,Acetoin的Km为3.60 mmol。 2.5 酶的底物特异性研究 在最适温度和最适pH下,分两个方向测定该酶的底物特异性,一种是测定每个酶氧化醇类物质的底物特异性,另一种是还原乙偶姻类物质的底物特异性。
葡萄糖 l 甘油醛-3.磷酸 丁二醇 乙酰磷酸乙醛 窿窿竹 图卜l典型的2,3一丁二醇代谢途径 metabolic Fig.1·1Representativepathwayof2,3.butanediol (1)EMP途径各种酶(2)乳酸脱氢酶(3)苹果酸脱氢酶 (4)丙酮酸.甲酸裂解酶 (5)甲酸裂解酶 (6)醛脱氢酶 (7)醇脱氢酶 (8磷酸乙酰转移酶 (9)乙酸激酶 (10)...
developmenturgentlyexploited.Biorefiningtechnology renewableresourcesasfeedstockis asone solution,inwhich proposed possible 2,3一butanediol fermentationisone issue.2.3-Butanediol,abulkchemical extensive important product,has inchemical andmedicine.Themetabolicof appHcations,especiallyprocesses,food,fuel pathway is ...
在微生物典型的2,3-BD合成途径中,糖类物质在α-乙酰乳酸合成酶 (α-acetolactate synthase, ALS)、α-乙酰乳酸脱羧酶 (α-acetolactate decarboxylase, ALDC)、2,3-丁二醇脱氢酶(2,3-butanediol dehydrogenase,2,3-BDH;又称乙偶姻还原酶,acetoin reductase,AR)的作用下经丙酮酸、α-乙酰乳酸、乙偶姻并最终...
微生物合成2,3-丁二醇的代谢工程 摘要 2,3-butanediol (2,3-BD), which is considered as an important microbial metabolite, has been widely used in many fields such as food, medicine, chemical, and so on. Microbial 2,3-BD production has a history of more than 100 years, but the low ...
Overflow metabolism-caused acetate accumulation is a major problem that restricts industrial applications of various bacteria. 2,3-Butanediol (2,3-BD) synthesis in microorganisms is an ancient metabolic process with unidentified functions. We demonstrate
cerevisiae进行遗传学改造,为实现生物燃料如乙醇、2,3-丁二醇(2,3-butanediol,2,3-BD)的工业化生产提供了保障 [2] 。 此方法的兴起不但能在保护生态环境的同时发展经济,实现能源结构的绿色转型,更是响应“十一五”发展规划、推动节能减排号召的一种有效手段。继乙醇之后,2,3-BD是一种性价比较高的新兴能源化合...