摘要: PROBLEM TO BE SOLVED: To produce a new (2S,3S)-2,3-butanediol dehydrogenase useful for the production of ketones and alcohols, especially an optically active vicinal diol.收藏 引用 批量引用 报错 分享 文库来源 其他来源 求助全文 (2S, 3S) -2,3- butanediol dehydrogenase 优质文献 ...
A NAD-dependent (R)-2,3-butanediol dehydrogenase (EC 1.1.1.4), selectively catalyzing the oxidation at the (R)-center of 2,3-butanediol irrespective of the absolute configuration of the other carbinol center, was isolated from cell extracts of the yeast Saccharomyces cerevisiae. Purification was...
To improve acetoin production, acetoin biosynthetic genes from Bacillus subtilis encoding α-acetolactate synthase (AlsS) and α-acetolactate decarboxylase (AlsD) were overexpressed, and BDH1 encoding butanediol dehydrogenase, which converts acetoin to 2,3-butanediol, was deleted. Furthermore, by NAD+ ...
Bacillus licheniformis is a potential 2,3-butanediol producer but the wild type strain (WX-02) produces a mix of D- and meso-type isomers. BudC in B. licheniformis is annotated as 2,3-butanediol dehydrogenase or acetoin reductase, but no pervious experiment was performed to verify this ...
Taking into consideration the substrate specificity of the YAL060W gene product, we propose the name of BDH for this gene. The corresponding enzyme is the first eukaryotic (2R, 3R)-2,3-butanediol dehydrogenase characterized of the medium-chain dehydrogenase/reductase family. 展开 ...
Introduction As a key enzyme in the microbial production of 2,3-butanediol, 2,3-butanediol dehydrogenase (also known as acetoin/diacetyl reductase) can reduce diacetyl to acetoin and then to 2,3-butanediol that has three stereoisomeric forms: meso-2,3-butanediol, (2R,3R)-2,3-butanediol, ...
medium-chain dehydrogenase/reductase SDR short-chain dehydrogenase/reductase PCR polymerase chain reaction BDH (2R,3R)-2,3-butanediol dehydrogenase GLC gas-liquid chromatography One of the tasks left after the completion of the various genome projects is to ascertain the function of the sequenced gen...
5-fold increase in (2R,3R)-2,3-butanediol dehydrogenase activity was observed in the total cell extracts. The isoelectric focusing pattern of the induced enzyme coincided with that of the pure BDH (pI 6.9). The disruption of the YAL060W gene was not lethal for the yeast under laboratory...
Mechanism for the formation of 2,3-butanediol stereoisomers in Bacillus polymyxa The mechanism of the formation of 2,3-butanediol isomers in Bacillus polymyxa was studied. We proposed a new model with NADPH-linked diacetyl reductase (S-acetoin forming) and R()-2,3-butanediol dehydrogenase. The...
Deletion of meso-2,3-butanediol dehydrogenase gene budC for enhanced D-2,3-butanediol production in Bacillus licheniformis BudC in B. licheniformis is annotated as 2,3-butanediol dehydrogenase or acetoin reductase, but no pervious experiment was performed to verify this ... G Qi,Y Kang,L Li...