We have synthesized a tritiated form of 2,22-dideoxyecdysone ((3H2-22,23)-3β,14α, 25-trihydroxy-5β-cholest-7-en-6-one) of high specific activity (2.2 TBq/mmol). We have examined the capacity of various endocrine (prothoracic glands, follicle cells) and peripheral (fat body, ...
Their structures were established to be (23S)-2,22-dideoxy-23-hydroxyecdysone and its 3-phosphate on the basis of spectroscopic analysis including 1H-NMR comparison with the known compounds, 2-deoxy-ecdysone and (23R)-and (23S)-23,25-dihydroxycholesterols.doi:10.1080/10575630008043767...
When incubated with larval brain-ring gland complexes, 5β-ketodiol was converted to ecdysone as well as to other metabolites, such as 22,25-dideoxyecdysone and 2-deoxyecdysone. Other larval tissues, namely the fat body, Malpighian tubules, gut and carcass are also able to transform 5β-...
The fragments were sequenced from both ends of the cloning site with M13 forward and reverse primers using the dideoxy chain termination method [89] on a PRISM 3700 automated DNA sequencer (PE Biosystems). Chromatogram traces were base-called with PHRED and assembled with PHRAP [90,91]. ...