CAGGAAGTAAGATGCCTGGAAC 下游:TGCAGCAGTACCAAGTTTAGTG 上游:AAGAGCAGCATGACCTCTCAC 下游:CTGCCTCAAGTCAGTGCCT 上游:AGAGAGCGATGCAGGAAACTT 下游:AAGGATGCCCACATCTTTTGG 上游:AAGAGGGATGCTGCCCTTAC 下游:TACGGCCAAATCCGTTCACA 筛选标准.使用 Cytoscape 软件构建"基因-中医症 状/现代医学症状"和"基因-中药"关联...
FDX1 上游:CCTCTTTGGAGTCTCTCGCGG 下游:CAGCCCAACCGTGATCTGT DLD DLAT GAPDH 上游:CTCTTGCTTTGGAATATGGAGC 下游:AAAAGCTTCTGATAAGGTCGGA 上游:ACTCCCCAGCCTTTAGCTC 下游:CAATCCCTTTCTCTACTGCCAAC 上游:GGAGCGAGATCCCTCCAAAAT 下游:GGCTGTTGTCATACTTCTCATGG dehydrogenase,GAPDH)为内参按 2−∆∆Ct 法分析....
下调HMGB2表达对肝癌LM3细胞上皮-间质转化的抑制作用及 其AKT/mTOR信号通路机制 魏雁虹1, 杨晨雪1, 杨广民1, 宋帅1, 李明1, 杨海娇1, 魏海峰2 （1. 吉林省人民医院医学检验中心，吉林长春130021；2. 吉林省人民医院生物治疗与基因诊断 重点实验室，吉林长春130021）［摘要］目的 目的：探讨下调肝癌细胞中高...
21. However, other studies reported that AMPK activation had a protumour function in RAS-transformed fibroblasts and astrocytes and promoted tumour cell survival22,23. AMPK can also alleviate cancer cell metabolic stress and apoptosis via mitochondrial pathways24,25. Therefore, the role of AMPK in ...
The human ACE2 transcript variant 2 was amplified using the OriGene primer set Forward: 5’-TCC ATT GGT CTT CTG TCA CCC G-3’ and Reverse: 5’-AGA CCA TCC ACC TCC ACT TCT C-3’. HPRT was used as the control housekeeping gene using primers, Forward: 5’-TCA GGC AGT ATA ATC CAA...
The pLKO.1-shRNAs targeting IRP2, SIRT1 and SIRT5 nucleotides were 5′- CCGGGGACCTAAATCAGAATCATAGCTCGAGCTATGATTCTGATTTAGGTCCTTTTTG-3′(sh-IRP2–1),5′-CCGGGCAAACATGTGTCCGGAATATCTCGAGATATTCCGGACACATGTTTGCTTTTTG-3′(sh-IRP2–2),5′-CCGGGCAAAGCCTTTCTGAATCTATCTCGAGATAGATTCAGAAAGGCTTTGC...
(5′–3′) AAGGGAATTGTGGCTCTGGG AGAGACTTCCAGCCAGTTGC GCCAACAAGTGGTATTCTCCA GTTGCTGGGGGAAGGAATGT AGGGCTACCATGCCAACTTC ACCATCGGGAATGAACGCTT GCAATGCTGAATCGTCCCAC TAAAGAAGGCCCTGTGGGTC ACGCTAGAGCAGATACCACG AACAGCAGAAGATTTCCCTGG CCGATCCAGACCTTCTCTGC CAGGGCTGCCTTCTCTTGTG Reverse (5′–3′) ...
ifseraetntrciebueftfeedcttsowthheicfhacctatnhaetxtphleailnowthliesvdeelcorfepas-epoinlaχrixzIxTezAd, see Supplementary Fig. S5(a). The large variation generated SH power for s-polarized fundamental bofeaχmzIxTxiAs close to the detection limit. Note that the visibility of the fringes ...
Second, weak localization is also dethuwhxpeiigectrthhliusuvrcFdednhdSiefesdfaamei,ngrnbeneiesensagocttsatauhsrtuirohovespaweepcyLρn.aeTMnsxix.onb(BRTtetee,h)scbidetacuuteoithnnsnteethsfioeretftiohreMsmledetRds.ooTitforFoheFfiicgFSRrt.edi a1r3o4,nm,en3ni2.seioioI-gsnfltaiprcqtotouiu...
(F: 5-’ TCAGGCGTCTGTAGAGGCTT-3’R: 5-’ ATGCACATCCTTCGATAAGACTG-3′), ZEB1 (F: 5-’ GATGATGAATGCGAGTCAGATGC-3’R: 5-’ ACAGCAGTGTCTTGTTGTTGT-3′), ZEB2 (F: 5-’ CAAGAGGCGCAAACAAGCC-3’R: 5-'GGTTGGCAATACCGTCATCC-3′), Vimentin (F: 5-’ GACGCCATCAACACCGAGTT-3’R...