英文名称:2×TSINGKE® Master qPCR Mix(SYBR Green I)总访问:1199 国产/进口:国产半年访问:96 产地/品牌:TSINGKE产品类别:分子生物学试剂 规格:TSE201最后更新:2024-3-28 货号: CAS 号: 参考报价:600元/5×1.0 mL 立即询价电话咨询 [发表评论][本类其他产品][本类其他供应商][收藏] ...
英文名2×TSINGKE Master qPCR Mix(SYBR Green I) 数量10000 供应商北京擎科生物科技有限公司 规格5×1ml 产品说明 2×TSINGKE® Master qPCR Mix(SYBR Green I) 【产品说明 】 本产品使用了抗体修饰的新型热启动Taq DNA Polymerase、精心优化的K+/NH4+Buffer、PCR增强剂、dNTPs、SYBR Green I荧光染料以及配套...
Striatin interacting protein 2 (STRIP2) is a core component of the striatin-interacting phosphatase and kinase (STRIPAK) complexes, which is involved in tumor initiation and progression via the regulation of cell contractile and metastasis. However, the
公司:北京擎科生物科技有限公司 联系人:张楠 联系电话: 18622657960 联系邮箱:zhangnan@tsingke.net 产品属性 保存条件 2xT5 Fast qPCR Mix(Probe) is shipped on Dry Iceand can be stored for up to 12 months at -20 ℃,or up to 2 weeks at 4℃. 保质期 2xT5 Fast qPCR Mix(Probe) is shipped ...
Total RNAs was extracted by TRIzol (Invitrogen) and cDNAs was synthesized using a Rever Ace qPCR RT Kit (TOYOBO). Real-time PCR was performed using SYBR Green, Real-time PCR Master Mix (Roche) and the ABI ViiA7 QPCR System (Applied Biosystems). ChIP-seq datasets were downloaded from the...
qRT–PCR was performed using the CFX96TM Real-Time System (Bio-Rad, California, USA) and 2 × TSINGKE® Master qPCR Mix (SYBR Green I) (TSINGKE, Beijing, China) reagents. Primer 6.0 was used to design PCR primers (Table S5). The cDNA was used as the template, and the β-...
Human PARP1 small interfering RNAs (siRNAs) were procured from TSINGKE (Beijing, China). The manufacturer’s protocol was followed for siRNA transfection, utilizing Lipofectamine RNAiMAX. Provided below are the siRNA target sequences: siVCAN-1: 5′-GATTGGTCAGGACTACAAA-3′; ...
a,bqPCR analysis ofIFNBexpression in H1299 cells stimulated with 500 ng/ml vRNA (3′ UTR) (a) or poly(I:C) (b), following a 24-h Dox-induced expression of N-mEGFP protein (n = 3 biologically independent samples).cImmunoblot analysis of the Dox-induced expression of NR203/...
The extracted RNA was used to synthesize cDNA using the ReverTra Ace qPCR RT Master Mix with gDNA Remover (TOYOBO, Japan) according to the manufacturer’s instructions. For miRNAs, reverse transcription was performed using the miRNA First Strand cDNA Synthesis Kit (Vazyme Biotech Co., Ltd., ...
In addition, ChIP-qPCR using anti-CREB antibody revealed that overexpression of LPCAT1 enhanced the binding of CREB to NRF1 promoter in Huh7 and knockdown of LPCAT1 inhibited that in MHCC97H, while ChIP-qPCR using IgG control had no such change (Fig. 5H). Together, these results indicate...