GeneCarer 2×Taq Advanced PCR Master Mix prevnext GeneCarer 2×Taq Advanced PCR Master Mix 货号:A301-01 规格:1×5mL; 价格:¥80 产品用途:常规PCR快速扩增 说明书
2× Rapid Taq Master Mix This product contains Taq DNA Polymerase, extension promoter, dNTP and optimized buffer system. The amplification speed can reach 15 sec/kb. It is suitable for rapid PCR reaction. The limit amplification speed within 1 kb can reach 1 sec/kb, which is large Save PCR...
适用于(应用)克隆, High-fidelity PCR 浓度2 U/µL 保真度(相对于 Taq)52 X 热启动内置热启动 反应次数500 Reactions 突出端平末端 反应形式独立 尺寸(最终产品)长达 20 kb 颜色Colorless Unit Size500 units 内容与储存 包括: •Phusion 热启动 II 高保真 DNA 聚合酶 (2 U/μL) ...
As discussed above, SARS-CoV-2 infection in older adult cultures led to an increase in basaloid-like 2 cells (Fig. 5a) associated with a pro-fibrotic state and epithelial–mesenchymal transition (EMT), including expression of ITGB6, VIM and KRT5 (Fig. 5b and Extended Data Fig. 1d). Typi...
RT-qPCRs were carried out using GoTaq qPCR Master mix (Promega, A6002), a CFX384 Real-Time System (Bio-Rad) and the following qPCR primers directed at the human PALB2 cDNA or the mouse control gene Pim1; human PALB2-Fw— 5’-GATTACAAGGATGACGACGATAAGATGGAC-3’, human PALB2-Rv...
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RT-PCR was performed with QuickTaq HS Dye Mix (Toyobo, Japan). The primer sequences which recognize AT2R and AT1R containing restriction enzyme sites are listed below. The primer recognition sites and primer sets are indicated in Supplemental Fig. 2 ((A) and (B)). The reaction conditions...
For shearing, cells were resuspended in 100ul chromatin sheering buffer (Active Motif) and transferred into wells of 96 well plate, sealed with PCR film (MiniAMP Optical Adhesive Film) and placed in PIXUL for shearing. PIXUL parameters were as follows: Cycles = 50; PRF = 1kHz; Burst = ...
Viral loads were determined by quantitative real-time PCR. Viral RNA was extracted by using a QIAamp vRNA Minikit (Qiagen, Germany). Reverse transcription was performed by using HiScript II Q RT SuperMix for qPCR (Vazyme, China). qPCR was conducted by using an Applied Biosystems QuantStudio ...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was first detected in late December 2019 and has spread worldwide. Coronaviruses are enveloped, positive sense, single-stranded RNA viruses and employ a complicated pattern of virus genome leng