First-strand cDNA was synthesized by the PrimeScript Reverse Transcriptase System (Takara, Japan). Full-length ORF primers of EoblGOBP2 were designed according to the nucleotide sequence of EoblGOBP2 (GenBank accession number: ACN29681) using Primer Premier 5.0 software (PREMIER Biosoft ...
Hu sheep (Ovis aries) is a rare white sheep breed, with four different types of lambskin patterns that have different values. However, the genetic mechanisms underlying different types of pattern formation remains unclear. This research aimed to characte
Reverse transcription of RNA coupled to amplification of the resulting cDNA by the polymerase chain reaction (RT-PCR) is one of the principal molecular technologies in use today, with applications across all areas of science and medicine. In its real-time, fluorescence-based usage (RT-qPCR), it...
Real-time polymerase chain reaction (qPCR) revealed higher levels of m-RNA for both receptors in the testis, ampulla, seminal vesicles, and vas deferens, than in the other organs of the reproductive tract (p < 0.05). Western blot analyses showed protein bands compatible with the MT1 in the...