Together these experiments indicated that the ITL1-TR2 oligonucleotide pair exhibited excellent qPCR performance, as shown in the Supplementary Methods and Table S2. Finally, each genomic DNA sample of the bacteriophage ɸITL-1 (purified from the contaminated sewage samples), standards, and controls...
gene was analyzed at each time point (24 h, 48 h and 6 days), using semi-quantitative real-time PCR, as already described [25]. Briefly, qPCR was performed using the GoTaq(R) qPCR Master Mix (Promega) and expression levels were normalized usingACTB(β-actin) as a reference ...
The titer was determined by qPCR (Real-time quantitative PCR) method. To be detailed, 2 μL of pseudotyped viruses containing the SARS-CoV-2 N gene fragment were used template and mixed with qPCR mix containing 10 μL of 2 × T5 Fast qPCR Mix, 0.8 μL of forward primer/reverse primer...
qPCR was performed with 1 µL of a primer mix (2 µM each of forward and reverse primer), 2.5 µL SYBR green (ThermoFisher), and 1.5 µL of cDNA. Master mixes of the primer mix + SYBR green were added to all applicable wells prior to the addition of cDNA, which was added...
SENSIFast Probe No ROX qPCR mix Bioline BIO-86005 TaqMan Gene Expression assay for IL-8 Life Technologies Cat #4331182 SYBR Green Quantitect assay for TNF (Hs_TNF_3_SG) QIAGEN Cat #QT01079561 NEBNext Ultra II directional kit New England Biolabs, NEB Cat # E7765 NEBNext Multiplex Oligos ...
In this experiment, we observed that 48 h after transfection with a mix of siCDX2 there was a decrease of about 50% in ST6GalNAc-I mRNA levels as well as in the CDX2 known targets β3Gal-T5 and LI- cad. However, 72 h after transfection, ST6GalNAc-I levels increased again, despite...
Glycosyltransferase mRNA expression levels were quantified by RT-qPCR using TaqManTM Universal PCR Master Mix II, no UNG, and the specific TaqManTM gene expression assays, listed below, in a 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA; Thermo...
qPCR was performed with 1 µL of a primer mix (2 µM each of forward and reverse primer), 2.5 µL SYBR green (ThermoFisher), and 1.5 µL of cDNA. Master mixes of the primer mix + SYBR green were added to all applicable wells prior to the addition of cDNA, which ...
Then, Goldenstar™ RT6 cDNA Synthesis Kit Ver.2 (Tsingke, Beijing, China) was used to synthesise RNA into cDNA; The TSE202 2 × T5 Fast qPCR Mix (SYBR Green I) (Tsingke, Beijing, China) was used to amplify the AIM2 gene, and it was quantified by real-time fluorescence ...
The fast-growing and non-pathogenic bacterial strain M. smegmatis mc2 155 serves as a more suitable model for obtaining spontaneous resistant mutants, studying bacterial resistance, and understanding the drug’s mechanism of action. We managed to procure spontaneous mutants of M. smegmatis mc2 155 ...