品名:iTaq™ Universal SYBR® Green Supermix 规格:2,500 x 20 µl rxns, 25 ml (5 x 5 ml) 产品描述: Use iTaq Universal SYBR Green Supermix with blended ROX and fluorescein passive reference dyes for dye-based quantitative PCR (qPCR). ...
iTaq Universal SYBR® Green Supermix Bio Rad Cat# 1725124 Vectastain Elite ABC kit Vector Lab Cat# PK-6011 Dual-Luciferase Reporter Assay System Promega Cat# E1910 ChIP-IT Express ChIP Kit Active Motif Cat# 53008 Pierce™ Anti-HA Magnetic Beads Thermo Fisher Cat# 88837 Anti-FLAG® M2-...
The relative expression levels of the SARS-CoV-2 nucleocapsid (N) protein were determined from the quantitative Real-time PCR (qRT-PCR) using the iTaq Universal One-Step RT-qPCR Kit (#1725151; Bio-Rad, Hercules, CA). Triplication of PCR reactions was included in all assays. The expression...
qPCR of RNA Abundance RNA was extracted using the Qiagen RNeasy Mini kit. cDNA was synthesized using a iScript kit (BioRad). The abundance of the cDNA was measured by qPCR using the iTaq Universal SYBR Green Supermix kit (BioRad), and run on the 7900HT Real Time PCR System or QuantSt...
Quantitative PCR was performed using the iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) with the primers listed in Table 2. The expression levels of all target genes were normalized to the GAPDH value. Table 2. Sequences of the primer pairs used for real-time quantitative...
Quantitative reverse PCR (qPCR) was performed with iTaq universal SYBR green supermix (Bio-Rad, Hercules, CA, USA) in the C1000 Touch Thermal cycler (Bio-Rad, Hercules, CA, USA). Data was analyzed using CFX manager 3.1 (BioRad, Hercules, CA, USA). Levels of GAPDH expression were used...
品名:iTaq™ Universal SYBR® Green Supermix 规格:5,000 x 20 µl rxns, 50 ml (10 x 5 ml) 产品描述: 5,000 x 20 µl reactions, 50 ml (10 x 5 ml), 2x qPCR mix, contains dNTPs, iTaq DNA Polymerase, MgCl2, SYBR® Green I, ...
RNA extraction, cDNA synthesis and qPCR Total RNA was isolated using Isolate II RNA Mini Kit (Bioline). cDNA was synthesized using SensiFAST cDNA Synthesis Kit (Bioline). Quantitative real time PCR was performed with iTaq Universal SYBR Green Supermix (BioRad) in a CFX96 Touch BioRad system...
The cDNAs were further analyzed by real-time qPCR using iTaq™ Universal SYBR Green® Supermix (Bio-Rad, 1725121) on a LightCycler® 96 Instrument (Roche, 05815916001). Chromatin immunoprecipitation The freshly dissected cerebral cortex was partially triturated in phosphate-buffered saline (PBS)...
RNA was reverse transcribed using iScript cDNA Synthesis kit (Bio-Rad), and qPCR was performed with iTaq Universal SYBR Green Supermix (Bio-Rad) on an Applied Biosystems 7300 Real-Time PCR system (Invitrogen). Specific primers are listed in Table S1. NGS data preprocessing FASTQ files from...