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第四代检测和HIV DNA PCR的窗口期大多在16天左右（多数介于15-20天），HIV RNA检测的窗口期大多在11...
Zip1+/+, Zip1+/− and Zip1−/− mice were identified using two pairs of PCR primers (Primer pair 1: F1: 5’-TCTCTGTATAGGTCCAAGAGTCAC-3’, R1: 5’-TGAAGGAGGGACAAGCAAATA. Primer pair 2: F2: 5’-CATGTGACGGTAAGCATTGACTTC-3’, R1: 5’-TGAAGGAGGGACAAGCAAATAAGTG-3’). ...
0.5 µl rt-PCR primer mix (40 µM F and R, 10 µM probe), 3.5 µl of H2O and 1 µl DNA template were used. The final concentration of each rt-PCR primer and probe in the reaction was 2 µM F and R and 0.5 µM probe. The cycling conditions...
Each amplification reaction was performed using 100 ng (2 μL) of genomic DNA in 25 μL of reaction mixture consisting of 1.0 μL of each primer (10 μmo1/L), 12.5 μL 2 × Taq PCRMasterMix (constituent: 0.1 U Taq polymerase/μL, 500 μM dNTP each, 20 mM Tris-HCl, pH 8.3,...
– PCR Read: 60°C 30 secPredenaturation: 95°C 10 minCycling: Denaturation: 95°C 15 secAnnealing and extension: 60°C 60 sec; 35xPost – PCR Read: 60°C 30 sec2x TaqMan Genotyping Master Mix40x specific TaqMan® SNP Genotyping Assay for rs4880 (C_8709053)0,5 μl (500 ng) ...
Using GoTaq qPCR master mix, we performed qRT-PCR with 2 to 3 technical replicates for each marker and reference gene (Table S3). Marker genes of chief cells were selected from a list of DEGs from murine chief cells in a scRNA-seq data recently published (Busslinger et al., 2021). ...
Bifidobacterium longum 105-A produces markedly high amounts of capsular polysaccharides (CPS) and exopolysaccharides (EPS) that should play distinct roles in bacterial–host interactions. To identify the biological function of B. longum 105-A CPS/EPS, we
Firstly, the effects on tenderness observed in the B × Y population are not nearly as large as those in the IMQP; the additive effects calculated for shear force and tenderness in the IMQP are nearly two and three times the size of those for the B × Y family, respectively (when ...
PCR products were denatured in Hi-Di formamide, pooled with LIZ 500 GS internal molecular weight control and loaded on an ABI 3130 genetic analyzer (Applied Biosystems, Carlsbad, California, USA). Fragments were measured and compared using Gene-Mapper software Version 4 (Applied Biosystems, ...