英文名称:ClonExpress MultiS One Step Cloning Kit testsClonExpress MultiS One Step Cloning Kit tests is available 2 times 来自 Vazyme labs,中文名称:ClonExpress多步一步克隆工具包测试ClonExpress多步一步克隆工具包测试可用2,产品货号:
Heavy-chain variable region and light-chain variable region of the recombinant antibody were amplified (2× Phanta Max Master Mix, Vazyme, P515-01) using the positive clones as the template. Heavy-chain variable region was fused (ClonExpress II One Step Cloning Kit, Vazyme, C112-01) into the...
Recombinant antibody heavy chain variable region and light chain variable region were amplified (2× Phanta Max Master Mix, Vazyme, P515-01) using the positive clones screened from the library as the template. Heavy chain variable region was fused (ClonExpress II One Step Cloning Kit, Vazyme,...
PCR product has an A at the 3’ end, which can be directly cloned into the T vector and is suitable for ClonExpress® rapid cloning kit (C112/C113/C114) Fast amplification speed: 15 sec/kb, the limit amplification speed can reach 1 sec/kb within 1 kb; Convenient operation: amplificati...
To construct the Nme2ABE8e plasmid, the reading frame encoding TadA-8e from ABE8e (Addgene:#138,489) [33] was amplified by PCR and used to replace the TadA-8.17 fragment within Nme2ABE8.17 [39] using a ClonExpress Ultra One Step Cloning Kit (Vazyme, Nanjing, China). TadA-8e DNA ...
so PCR products can be directly loaded for electro_x0002_phoresis after the reaction, which is convenient to use. The PCR product has an adenine at the 3' end that can be cloned into the T vector. It is also compatible with ClonExpress and TOPO cloning kits (Vazyme #C112/C113/C115/C6...
Thoracic aortic aneurysm (TAA) is a localized or diffuse dilatation of the thoracic aortas, and causes many sudden deaths each year worldwide. However, there is no effective pharmacologic therapy. Here, we show that AGGF1 effectively blocks TAA-associated arterial inflammation and remodeling in thre...
567 (C10310-1) were supplied by Dojindo (Japan) and RiboBio (P.R. China.), respectively. HiScript II Q RT SuperMix for qPCR (R222-01), 2× Taq Master Mix (P212-01), and ClonExpress Ultra One Step Cloning Kit (C115-01) were obtained from Vazyme (P.R. China.). Inhibitors ...
The chromatin organization modifier domain (chromodomain) is an evolutionally conserved motif across eukaryotic species. The chromodomain mainly functions as a histone methyl-lysine reader to modulate gene expression, chromatin spatial conformation and g
Dysregulated expression of splicing factors has important roles in cancer development and progression. However, it remains a challenge to identify the cancer-specific splicing variants. Here we demonstrate that spliceosome component BUD31 is increased in